BARD1 Participates with BRCA1 in Homology-directed Repair of Chromosome Breaks

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 2003 Oct 16

PMID 14560035

Citations 58

Authors

Ulrica K Westermark

Marsha Reyngold

Adam B Olshen

Richard Baer

Maria Jasin

Mary Ellen Moynahan

Affiliations

Soon will be listed here.

Abstract

The BRCA1 tumor suppressor has been implicated in the maintenance of chromosomal stability through homology-directed repair of DNA double-strand breaks. Much of the BRCA1 in cells forms a heterodimeric complex with a structurally related protein BARD1. We report that expression of truncated mouse or human BARD1 peptides capable of interacting with Brca1 results in a homologous-repair deficiency. Repair is mildly reduced in Brca1 wild-type cells and severely reduced in cells that harbor a Brca1 splice product deleted for exon 11. Nuclear localization of the Brca1 or BARD1 peptides is not compromised, implying that the repair deficiency is caused by a more direct effect on repair. The tumor suppressor activity of BRCA1 may require the participation of BARD1 to maintain chromosome integrity through the homologous-repair pathway.

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