Absence of the Thermal Transition in Apo-alpha-lactalbumin in the Molten Globule State. A Study by Differential Scanning Microcalorimetry

Overview

Journal J Mol Biol

Publisher Elsevier

Specialties Microbiology
Molecular Biology

Date 1992 Nov 20

PMID 1453446

Citations 18

Authors

K Yutani

K Ogasahara

K Kuwajima

Affiliations

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Abstract

To estimate the energy level of the molten globule state, the heat capacity function of apo-alpha-lactalbumin in the molten globule state has been examined using a scanning microcalorimeter at neutral pH. The results showed that the enthalpy difference between the molten globule state and presumed unfolded state by heating was almost zero at neutral pH, demonstrating that the molten globule state does not exhibit any co-operative transition upon heating. This is in agreement with the results already reported at acid pH, but is apparently in conflict with that recently reported with some assumptions at neutral pH.

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