Production of Monoclonal Antibody to a Phenolic Glycolipid of Mycobacterium Tuberculosis and Its Use in Detection of the Antigen in Clinical Isolates

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1992 Dec 1

PMID 1452686

Citations 6

Authors

S N Cho

J S Shin

M Daffe

Y Chong

S K Kim

J D Kim

Affiliations

Soon will be listed here.

Abstract

A monoclonal antibody (MAbIII604) specific to phenolic glycolipid Tb (PGL-Tb), a Mycobacterium tuberculosis-specific antigen, was produced and used in the detection of the antigen. MAbIII604 reacted with the PGL-Tb antigen but not with other phenolic glycolipids from Mycobacterium leprae, M. bovis, and M. kansasii, thus indicating the specificity of the monoclonal antibody to PGL-Tb. A dot enzyme-linked immunosorbent assay with MAbIII604 was employed to detect the PGL-Tb antigen in lipids purified from M. tuberculosis clinical isolates. Of 50 isolates, 32 (64.0%) showed clear evidence of the PGL-Tb antigen by the dot enzyme-linked immunosorbent assay, but there were marked variations in the intensities and sizes of spots. This suggests differences in PGL-Tb antigen production among M. tuberculosis strains even when they are grown in the same culture media and conditions. This was most evident from the fact that in only eight (16.0%) of the isolates examined was the PGL-Tb antigen detectable by thin-layer chromatography, which is much less sensitive for the detection of glycolipid antigens. This study shows that monoclonal antibodies specific to PGL-Tb are useful in detecting the antigen in lipid extracts and that there is a marked variation in the PGL-Tb production among M. tuberculosis clinical isolates.

Citing Articles

Anti-phenolic glycolipid antibodies in infected cattle.

Zhou Z, van Hooij A, van Dijk J, Musch N, Pierneef L, Khalid H One Health. 2025; 20:100982.

PMID: 39974705 PMC: 11835577. DOI: 10.1016/j.onehlt.2025.100982.

Underestimated Manipulative Roles of Cell Envelope Glycolipids During Infection.

Garcia-Vilanova A, Chan J, Torrelles J Front Immunol. 2020; 10:2909.

PMID: 31921168 PMC: 6930167. DOI: 10.3389/fimmu.2019.02909.

Genetics of Capsular Polysaccharides and Cell Envelope (Glyco)lipids.

Daffe M, Crick D, Jackson M Microbiol Spectr. 2014; 2(4).

PMID: 25485178 PMC: 4255156. DOI: 10.1128/microbiolspec.MGM2-0021-2013.

The phenolic glycolipid of Mycobacterium tuberculosis differentially modulates the early host cytokine response but does not in itself confer hypervirulence.

Sinsimer D, Huet G, Manca C, Tsenova L, Koo M, Kurepina N Infect Immun. 2008; 76(7):3027-36.

PMID: 18443098 PMC: 2446685. DOI: 10.1128/IAI.01663-07.

Cytometric detection of mycobacterial surface antigens: exposure of mannosyl epitopes and of the arabinan segment of arabinomannans.

Ozanne V, Vercellone A, Fournie J, Daffe M J Bacteriol. 1996; 178(24):7254-9.

PMID: 8955410 PMC: 178641. DOI: 10.1128/jb.178.24.7254-7259.1996.

References

Andersen A, HANSEN E . Structure and mapping of antigenic domains of protein antigen b, a 38,000-molecular-weight protein of Mycobacterium tuberculosis. Infect Immun. 1989; 57(8):2481-8. PMC: 313474. DOI: 10.1128/iai.57.8.2481-2488.1989. View

Papa F, David H . The use of PGL-Tb 1 from M. tuberculosis in the serological diagnosis of tuberculous meningitis: a preliminary report. Acta Leprol. 1989; 7 Suppl 1:136-7. View

Kingston A, Salgame P, Mitchison N, Colston M . Immunological activity of a 14-kilodalton recombinant protein of Mycobacterium tuberculosis H37Rv. Infect Immun. 1987; 55(12):3149-54. PMC: 260041. DOI: 10.1128/iai.55.12.3149-3154.1987. View

Young D, Harnisch J, Knight J, Buchanan T . Detection of phenolic glycolipid I in sera from patients with lepromatous leprosy. J Infect Dis. 1985; 152(5):1078-81. DOI: 10.1093/infdis/152.5.1078. View

Ivanyi J, Krambovitis E, Keen M . Evaluation of a monoclonal antibody (TB72) based serological test for tuberculosis. Clin Exp Immunol. 1983; 54(2):337-45. PMC: 1535890. View

Kolk A, Ho M, Klatser P, Eggelte T, Kuijper S, de Jonge S . Production and characterization of monoclonal antibodies to Mycobacterium tuberculosis, M. bovis (BCG) and M. leprae. Clin Exp Immunol. 1984; 58(3):511-21. PMC: 1577099. View

Hawkes R, Niday E, Gordon J . A dot-immunobinding assay for monoclonal and other antibodies. Anal Biochem. 1982; 119(1):142-7. DOI: 10.1016/0003-2697(82)90677-7. View

Papa F, Luquin M, David H . DOT-ELISA for detection of phenolic glycolipid PGL-Tb1 and diacyl-trehalose antigens of Mycobacterium tuberculosis. Res Microbiol. 1992; 143(3):327-31. DOI: 10.1016/0923-2508(92)90024-i. View

Lee T, Cho S, Yoon K, Shin J, Kim J . Comparison of DNA fragment patterns between the phenolic glycolipid-Tb producers and non-producers of Mycobacterium tuberculosis. Yonsei Med J. 1991; 32(3):243-9. DOI: 10.3349/ymj.1991.32.3.243. View

10.

Daffe M, Cho S, Chatterjee D, Brennan P . Chemical synthesis and seroreactivity of a neoantigen containing the oligosaccharide hapten of the Mycobacterium tuberculosis-specific phenolic glycolipid. J Infect Dis. 1991; 163(1):161-8. DOI: 10.1093/infdis/163.1.161. View

11.

Cho S, Cellona R, Fajardo Jr T, Abalos R, Cruz E, Walsh G . Detection of phenolic glycolipid-I antigen and antibody in sera from new and relapsed lepromatous patients treated with various drug regimens. Int J Lepr Other Mycobact Dis. 1991; 59(1):25-31. View

12.

Olds G, Sanson A, Daniel T . Characterization of Mycobacterium tuberculosis antigen 5 epitopes by using a panel of 19 monoclonal antibodies. J Clin Microbiol. 1987; 25(3):471-5. PMC: 265954. DOI: 10.1128/jcm.25.3.471-475.1987. View

13.

Baird P, Hall L, Coates A . Cloning and sequence analysis of the 10 kDa antigen gene of Mycobacterium tuberculosis. J Gen Microbiol. 1989; 135(4):931-9. DOI: 10.1099/00221287-135-4-931. View

14.

Martin Casabona N, Gonzalez Fuente T, Arcalis Arce L, Otal Entraigas J, Vidal Pla R . Evaluation of a phenolglycolipid antigen (PGL-Tb 1) from M. tuberculosis in the serodiagnosis of tuberculosis: comparison with PPD antigen. Acta Leprol. 1989; 7 Suppl 1:89-93. View

15.

Papa F, Laszlo A, David H, Daffe M . Serological specificity of Mycobacterium tuberculosis glycolipids. Acta Leprol. 1989; 7 Suppl 1:98-101. View

16.

Neill M, Klebanoff S . The effect of phenolic glycolipid-1 from Mycobacterium leprae on the antimicrobial activity of human macrophages. J Exp Med. 1988; 167(1):30-42. PMC: 2188820. DOI: 10.1084/jem.167.1.30. View

17.

Kadival G, Chaparas S . Production, characterization, and species specificity of five monoclonal antibodies to Mycobacterium tuberculosis. J Clin Microbiol. 1987; 25(1):76-80. PMC: 265827. DOI: 10.1128/jcm.25.1.76-80.1987. View

18.

Daffe M, LACAVE C, Laneelle M, LANEELLE G . Structure of the major triglycosyl phenol-phthiocerol of Mycobacterium tuberculosis (strain Canetti). Eur J Biochem. 1987; 167(1):155-60. DOI: 10.1111/j.1432-1033.1987.tb13317.x. View

19.

David H, Papa F . Preliminary evaluation of a Mycobacterium tuberculosis phenolglycolipid antigen in the serologic diagnosis of tuberculosis. Ann Inst Pasteur Microbiol. 1988; 139(3):289-94. DOI: 10.1016/0769-2609(88)90020-8. View

20.

Cho S, Yanagihara D, Hunter S, GELBER R, Brennan P . Serological specificity of phenolic glycolipid I from Mycobacterium leprae and use in serodiagnosis of leprosy. Infect Immun. 1983; 41(3):1077-83. PMC: 264610. DOI: 10.1128/iai.41.3.1077-1083.1983. View