Gliadin Uptake in Human Enterocytes. Differences Between Coeliac Patients in Remission and Control Individuals

Overview

Journal Gut

Specialty Gastroenterology

Date 1992 Nov 1

PMID 1452073

Citations 9

Authors

S Friis

E Dabelsteen

H Sjostrom

O Noren

S Jarnum

Affiliations

Soon will be listed here.

Abstract

The pepsin trypsin digest of the wheat prolamin gliadin (PT-gliadin) is deleterious to the small intestinal mucosa of coeliac patients. The handling of PT-gliadin by the intestinal epithelium in coeliac patients in remission and control individuals was investigated by in vivo instillation of PT-gliadin. The uptake of PT-gliadin was monitored by immunofluorescence microscopy of intestinal biopsy specimens, using affinity purified PT-gliadin antibodies. Control individuals show weak staining in the apical region of the enterocytes thereby showing an uptake of PT-gliadin. Coeliac patients have a conspicuous fluorescence in relation to the lateral membrane/intercellular space of enterocytes and intense staining intracellularly in the apical region. There is only weak staining in the enterocytes after the instillation was terminated, indicating an intracellular clearance. The study shows that normal enterocytes are able to take up PT-gliadin. The increased uptake in coeliac patients might be of importance for the pathogenesis either by direct toxicity or by presentation to immunocompetent cells. Furthermore, the results are in agreement with the suggestion of a functional alteration in the zonula occludens in the intestinal epithelium of coeliac patients.

Citing Articles

Gliadin peptide P31-43 localises to endocytic vesicles and interferes with their maturation.

Barone M, Nanayakkara M, Paolella G, Maglio M, Vitale V, Troiano R PLoS One. 2010; 5(8):e12246.

PMID: 20805894 PMC: 2923621. DOI: 10.1371/journal.pone.0012246.

Visualization of transepithelial passage of the immunogenic 33-residue peptide from alpha-2 gliadin in gluten-sensitive macaques.

Mazumdar K, Alvarez X, Borda J, Dufour J, Martin E, Bethune M PLoS One. 2010; 5(4):e10228.

PMID: 20419103 PMC: 2856682. DOI: 10.1371/journal.pone.0010228.

Noninflammatory gluten peptide analogs as biomarkers for celiac sprue.

Bethune M, Crespo-Bosque M, Bergseng E, Mazumdar K, Doyle L, Sestak K Chem Biol. 2009; 16(8):868-81.

PMID: 19716477 PMC: 3721637. DOI: 10.1016/j.chembiol.2009.07.009.

Antigen presentation in celiac disease.

Qiao S, Sollid L, Blumberg R Curr Opin Immunol. 2009; 21(1):111-7.

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Interferon-gamma released by gluten-stimulated celiac disease-specific intestinal T cells enhances the transepithelial flux of gluten peptides.

Bethune M, Siegel M, Howles-Banerji S, Khosla C J Pharmacol Exp Ther. 2009; 329(2):657-68.

PMID: 19218531 PMC: 2672868. DOI: 10.1124/jpet.108.148007.

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