Incorporation of Tritiated Cytidine into Ribonucleic Acid by Isolated Pea Nuclei

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1962 Aug 1

PMID 14491776

Citations 9

Authors

J H Rho

M I Chipchase

Affiliations

Soon will be listed here.

Abstract

A new method for the isolation of the nuclei from plant tissue has been devised. This method consists in passing the plant tissue through a set of spring-loaded, counter-rotating rollers and collecting the liberated nuclei in sucrose solution containing calcium ions. A semi-automatic machine which couples the rollers with a special tissue chopping device has been constructed. It has been shown that nuclei obtained in this way actively incorporate cytidine-H(3) into RNA. This incorporation is increased in the presence of nucleoside triphosphates and an energy-regenerating system.

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Isolation of nuclei from rice tissue grown in suspension culture.

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Chromatin- and nuclei-directed ribonucleic Acid synthesis in sugar beet root.

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SYNTHESIS OF TRANSFER RNA BY ISOLATED NUCLEI.

Chipchase M, Birnstiel M Proc Natl Acad Sci U S A. 1963; 49(5):692-9.

PMID: 16591090 PMC: 299955. DOI: 10.1073/pnas.49.5.692.

ISOLATION OF THE GOLGI APPARATUS FROM PLANT CELLS.

Morre D, Mollenhauer H J Cell Biol. 1964; 23:295-305.

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ON THE NATURE OF NUCLEOLAR RNA.

Chipchase M, Birnstiel M Proc Natl Acad Sci U S A. 1963; 50:1101-7.

PMID: 14096184 PMC: 221280. DOI: 10.1073/pnas.50.6.1101.

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