Isolation and Characterization of an Anaerobic Chlorophenol-transforming Bacterium

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1992 Sep 1

PMID 1444400

Citations 31

Authors

T Madsen

D Licht

Affiliations

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Abstract

An obligately anaerobic bacterium which transforms several chlorinated phenols was isolated. Dechlorination of the substituents ortho to the phenolic OH group was preferred, while removal of a meta-substituted chlorine was observed only with 3,5-dichlorophenol. The bacterium was a gram-positive, endospore-forming, motile, slightly curved rod. Sulfate was not reduced. Nitrate was reduced via nitrite to ammonium. The bacterium is related to the genus Clostridium. The highest growth rate was obtained in a medium containing pyruvate and yeast extract. Pyruvate supported growth as the sole source of carbon, and the fermentation of pyruvate produced almost equimolar amounts of acetate.

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