A CDNA Clone for a Novel Nuclear Protein with DNA Binding Activity

Overview

Journal Chromosoma

Specialty Molecular Biology

Date 1992 Oct 1

PMID 1424986

Citations 11

Authors

T Mattioni

C R Hume

S Konigorski

P Hayes

Z Osterweil

J S Lee

Affiliations

Soon will be listed here.

Abstract

In an effort to identify trans-acting factors regulating specific genes, we cloned a novel human gene, DBP-5. The cDNA clone contains a predicted open reading frame coding for a potential 1,179 amino acid protein. The mRNA corresponding to DBP-5 is ubiquitously distributed, and the gene is phylogenetically conserved. Immunofluorescence analyses with several cell lines indicate that the protein is localized to the nucleus. Sequence analysis revealed unusual features of the predicted protein structure, including four completely conserved repeats. The phylogenetic conservation of DBP-5, the ubiquity of its expression, its nuclear localization, and its ability to bind DNA sequences, raise the possibility that DBP-5 may play a role in the organization of interphase chromatin and/or in transcriptional regulation.

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References

Dale R, McClure B, Houchins J . A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 S rDNA. Plasmid. 1985; 13(1):31-40. DOI: 10.1016/0147-619x(85)90053-8. View

Reith W, Barras E, Satola S, Kobr M, Reinhart D, Sanchez C . Cloning of the major histocompatibility complex class II promoter binding protein affected in a hereditary defect in class II gene regulation. Proc Natl Acad Sci U S A. 1989; 86(11):4200-4. PMC: 287418. DOI: 10.1073/pnas.86.11.4200. View

Klemsz M, McKercher S, Celada A, Van Beveren C, Maki R . The macrophage and B cell-specific transcription factor PU.1 is related to the ets oncogene. Cell. 1990; 61(1):113-24. DOI: 10.1016/0092-8674(90)90219-5. View

Hume C, Shookster L, Collins N, OReilly R, Lee J . Bare lymphocyte syndrome: altered HLA class II expression in B cell lines derived from two patients. Hum Immunol. 1989; 25(1):1-11. DOI: 10.1016/0198-8859(89)90065-7. View

Johnson P, McKnight S . Eukaryotic transcriptional regulatory proteins. Annu Rev Biochem. 1989; 58:799-839. DOI: 10.1146/annurev.bi.58.070189.004055. View

Liou H, Boothby M, Glimcher L . Distinct cloned class II MHC DNA binding proteins recognize the X box transcription element. Science. 1988; 242(4875):69-71. DOI: 10.1126/science.3140376. View

Gladstone P, Pious D . Identification of a trans-acting function regulation HLA-DR expression in a DR-negative B cell variant. Somatic Cell Genet. 1980; 6(2):285-98. DOI: 10.1007/BF01538802. View

Bull M, van Hoef A, Gorski J . Transcription analysis of class II human leukocyte antigen genes from normal and immunodeficient B lymphocytes, using polymerase chain reaction. Mol Cell Biol. 1990; 10(7):3792-6. PMC: 360836. DOI: 10.1128/mcb.10.7.3792-3796.1990. View

Hume C, Lee J . Congenital immunodeficiencies associated with absence of HLA class II antigens on lymphocytes result from distinct mutations in trans-acting factors. Hum Immunol. 1989; 26(4):288-309. DOI: 10.1016/0198-8859(89)90007-4. View

10.

Mirkovitch J, Mirault M, Laemmli U . Organization of the higher-order chromatin loop: specific DNA attachment sites on nuclear scaffold. Cell. 1984; 39(1):223-32. DOI: 10.1016/0092-8674(84)90208-3. View

11.

Seed B, Aruffo A . Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure. Proc Natl Acad Sci U S A. 1987; 84(10):3365-9. PMC: 304871. DOI: 10.1073/pnas.84.10.3365. View

12.

Nakano M, Tobita T, Ando T . Studies on a protamine (galline) from fowl sperm. 3. The total amino acid sequence of intact galline molecule. Int J Pept Protein Res. 1976; 8(6):565-78. View

13.

Higgins R, Dahmus M . Rapid visualization of protein bands in preparative SDS-polyacrylamide gels. Anal Biochem. 1979; 93(2):257-60. DOI: 10.1016/s0003-2697(79)80148-7. View

14.

Macejak D, Sarnow P . Internal initiation of translation mediated by the 5' leader of a cellular mRNA. Nature. 1991; 353(6339):90-4. DOI: 10.1038/353090a0. View

15.

Mitchell P, Tjian R . Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins. Science. 1989; 245(4916):371-8. DOI: 10.1126/science.2667136. View

16.

Dieckmann C, Tzagoloff A . Assembly of the mitochondrial membrane system. CBP6, a yeast nuclear gene necessary for synthesis of cytochrome b. J Biol Chem. 1985; 260(3):1513-20. View

17.

Jackson R, Standart N . Do the poly(A) tail and 3' untranslated region control mRNA translation?. Cell. 1990; 62(1):15-24. DOI: 10.1016/0092-8674(90)90235-7. View

18.

Struhl K . Helix-turn-helix, zinc-finger, and leucine-zipper motifs for eukaryotic transcriptional regulatory proteins. Trends Biochem Sci. 1989; 14(4):137-40. DOI: 10.1016/0968-0004(89)90145-X. View

19.

Erlich H, Lee J, Petersen J, Bugawan T, Demars R . Molecular analysis of HLA class I and class II antigen loss mutants reveals a homozygous deletion of the DR, DQ, and part of the DP region: implications for class II gene order. Hum Immunol. 1986; 16(2):205-19. DOI: 10.1016/0198-8859(86)90049-2. View

20.

Singh H, Lebowitz J, Baldwin Jr A, Sharp P . Molecular cloning of an enhancer binding protein: isolation by screening of an expression library with a recognition site DNA. Cell. 1988; 52(3):415-23. DOI: 10.1016/s0092-8674(88)80034-5. View