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LIFE CYCLE ANALYSIS OF MAMMALIAN CELLS. II. CELLS FROM THE CHINESE HAMSTER OVARY GROWN IN SUSPENSION CULTURE

Overview
Journal Biophys J
Publisher Cell Press
Specialty Biophysics
Date 1964 Nov 1
PMID 14232130
Citations 18
Authors
T T PUCK
P Sanders
D Petersen
Affiliations
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Abstract

A method for life cycle analysis in mammalian cells which utilizes the collection function has been applied to the Chinese hamster ovary grown in suspension. The following durations were found for the various parts of the life cycle: S, 4.13 hours; G1, 4.71 hours; G2, 2.81 hours; mitosis, 0.81 hours. The cell has a total generation time of 12.4 hours as opposed to 20.1 hours for the S3 HeLa cell. However, the relative lengths of each phase of the life cycle are identical within experimental uncertainty in the two cells.

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References
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Tjio J, PUCK T . Genetics of somatic mammalian cells. II. Chromosomal constitution of cells in tissue culture. J Exp Med. 1958; 108(2):259-68. PMC: 2136870. DOI: 10.1084/jem.108.2.259. View
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PUCK T, CIECIURA S, Robinson A . Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects. J Exp Med. 1958; 108(6):945-56. PMC: 2136918. DOI: 10.1084/jem.108.6.945. View
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PUCK T, Steffen J . LIFE CYCLE ANALYSIS OF MAMMALIAN CELLS. I. A METHOD FOR LOCALIZING METABOLIC EVENTS WITHIN THE LIFE CYCLE, AND ITS APPLICATION TO THE ACTION OF COLCEMIDE AND SUBLETHAL DOSES OF X-IRRADIATION. Biophys J. 1963; 3:379-97. PMC: 1366456. DOI: 10.1016/s0006-3495(63)86828-9. View