DISTRIBUTION OF RADIOACTIVITY IN AUTOLYZED CELL WALL OF BACILLUS CEREUS DURING SPHEROPLAST FORMATION

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1964 Mar 1

PMID 14127573

Citations 3

Authors

D P KRONISH

R R Mohan

B S Schwartz

Affiliations

Soon will be listed here.

Abstract

Kronish, Donald P. (Warner-Lambert Research Institute, Morris Plains, N.J.), Raam R. Mohan, and Benjamin S. Schwartz. Distribution of radioactivity in autolyzed cell wall of Bacillus cereus during spheroplast formation. J. Bacteriol. 87:581-587. 1964.-Spheroplasts of Bacillus cereus strain T were produced from cells grown in the presence of uniformly labeled C(14)-glucose. At regular intervals during spheroplast formation, enzymatically degraded cell wall was isolated by a new procedure. Radioactivity of solubilized cell wall in cell-free material increased from 2.5 to 42% of the total incorporated label during spheroplast formation. The rate of cell-wall degradation as measured by increase in radioactivity was biphasic with relative slopes of 2.0 and 5.0. During autolytic depolymerization of B. cereus cell wall, two major components were solubilized at different rates. Chemical fractionation revealed these to be a peptide and a mucopeptide. The possibility of two enzymes being involved in spheroplast formation and cell-wall degradation is discussed.

Citing Articles

Effects of m-Tyrosine on Growth and Sporulation of Bacillus Species.

Aronson J, Wermus G J Bacteriol. 1965; 90(1):38-46.

PMID: 16562040 PMC: 315591. DOI: 10.1128/jb.90.1.38-46.1965.

Symposium on the fine structure and replication of bacteria and their parts. IV. Unbalanced cell-wall synthesis: autolysis and cell-wall thickening.

SHOCKMAN G Bacteriol Rev. 1965; 29(3):345-58.

PMID: 5318117 PMC: 441282. DOI: 10.1128/br.29.3.345-358.1965.

Autolytic mechanism for spheroplast formation in Bacillus cereus and Escherichia coli.

Mohan R, KRONISH D, PIANOTTI R, Epstein R, Schwartz B J Bacteriol. 1965; 90(5):1355-64.

PMID: 4954555 PMC: 315824. DOI: 10.1128/jb.90.5.1355-1364.1965.

References

Dark F, STRANGE R . Bacterial protoplasts from Bacillus species by the action of autolytic enzymes. Nature. 1957; 180(4589):759-60. DOI: 10.1038/180759a0. View

Mitchell P, Moyle J . Autolytic release and osmotic properties of protoplasts from Staphylococcus aureus. J Gen Microbiol. 1957; 16(1):184-94. DOI: 10.1099/00221287-16-1-184. View

Park J, Hancock R . A fractionation procedure for studies of the synthesis of cell-wall mucopeptide and of other polymers in cells of Staphylococcus aureus. J Gen Microbiol. 1960; 22:249-58. DOI: 10.1099/00221287-22-1-249. View

WORK E . The mucopeptides of bacterial cell walls. A review. J Gen Microbiol. 1961; 25:169-89. DOI: 10.1099/00221287-25-2-167. View

Salton M, Ghuysen J . [Effect of actinomycetin on bacterial cell walls]. Biochim Biophys Acta. 1957; 24(1):160-73. DOI: 10.1016/0006-3002(57)90159-2. View

Greenberg R, HALVORSON H . Studies on an autolytic substance produced by an aerobic sporeforming bacterium. J Bacteriol. 1955; 69(1):45-50. PMC: 357465. DOI: 10.1128/jb.69.1.45-50.1955. View

LEDERBERG J, St Clair J . Protoplasts and L-type growth of Escherichia coli. J Bacteriol. 1958; 75(2):143-60. PMC: 290050. DOI: 10.1128/jb.75.2.143-160.1958. View

Salton M . Studies of the bacterial cell wall. IV. The composition of the cell walls of some Gram-positive and Gram-negative bacteria. Biochim Biophys Acta. 1953; 10(4):512-23. DOI: 10.1016/0006-3002(53)90296-0. View

RONDLE C, Morgan W . The determination of glucosamine and galactosamine. Biochem J. 1955; 61(4):586-9. PMC: 1215838. DOI: 10.1042/bj0610586. View

10.

HOSODA J, Nomura M . Nature of the primary action of the autolysin of Bacillus subtilis. J Bacteriol. 1956; 72(5):573-81. PMC: 357962. DOI: 10.1128/jb.72.5.573-581.1956. View

11.

SHOCKMAN G, Kolb J, TOENNIES G . Relations between bacterial cell wall synthesis, growth phase, and autolysis. J Biol Chem. 1958; 230(2):961-77. View

12.

STRANGE R, Dark F . Cell-wall lytic enzymes at sporulation and spore germination in Bacillus species. J Gen Microbiol. 1957; 17(2):525-37. DOI: 10.1099/00221287-17-2-525. View

13.

WORK E . The action of a lytic enzyme from spores of a bacillus on various species of bacteria. Ann Inst Pasteur (Paris). 1959; 96(4):468-80. View

14.

Salton M, PAVLIK J . Studies of the bacterial cell wall. VI. Wall composition and sensitivity to lysozyme. Biochim Biophys Acta. 1960; 39:398-407. DOI: 10.1016/0006-3002(60)90191-8. View