Scanning Electron Microscopic Evaluation of the Arthritis in MRL/lpr Mice

Overview

Journal Rheumatol Int

Specialty Rheumatology

Date 1992 Jan 1

PMID 1411091

Authors

F X OSullivan

W J Koopman

S Gay

Affiliations

Soon will be listed here.

Abstract

The articular surfaces of disarticulated knee joints from MRL/lpr and MRL/n mice, aged 4-33 weeks were examined by light microscopy (LM) and scanning electron microscopy (SEM). Light microscopy did not reliably predict SEM findings. Most of the abnormalities detected by SEM were related to surface disruption of articular cartilage. However, areas of articular cartilage covered by tightly adherent non-confluent monolayers of stellate-shaped cells with intertwining cytoplasmic processes were observed. In these areas the integrity of the underlying cartilage matrix was disrupted, with exposure of collagen fibers. These findings suggested that outgrowth of proliferating synovial cells in the joints of arthritic MRL/lpr mice may lead to cartilage destruction.

References

OConnor P, Oates K, Gardner D, Middleton J, Orford C, Brereton J . Low temperature and conventional scanning electron microscopic observations of dog femoral condylar cartilage surface after anterior cruciate ligament division. Ann Rheum Dis. 1985; 44(5):321-7. PMC: 1001640. DOI: 10.1136/ard.44.5.321. View

Allard S, Bayliss M, Maini R . The synovium-cartilage junction of the normal human knee. Implications for joint destruction and repair. Arthritis Rheum. 1990; 33(8):1170-9. DOI: 10.1002/art.1780330818. View

Gilkeson G, Ruiz P, Grudier J, Kurlander R, Pisetsky D . Genetic control of inflammatory arthritis in congenic lpr mice. Clin Immunol Immunopathol. 1989; 53(3):460-74. DOI: 10.1016/0090-1229(89)90008-1. View

Andrews B, Eisenberg R, Theofilopoulos A, Izui S, Wilson C, McCONAHEY P . Spontaneous murine lupus-like syndromes. Clinical and immunopathological manifestations in several strains. J Exp Med. 1978; 148(5):1198-215. PMC: 2185049. DOI: 10.1084/jem.148.5.1198. View

Tanaka A, OSullivan F, Koopman W, Gay S . Etiopathogenesis of rheumatoid arthritis-like disease in MRL/1 mice: II. Ultrastructural basis of joint destruction. J Rheumatol. 1988; 15(1):10-6. View

Dayer J, Krane S, Russell R, Robinson D . Production of collagenase and prostaglandins by isolated adherent rheumatoid synovial cells. Proc Natl Acad Sci U S A. 1976; 73(3):945-9. PMC: 336037. DOI: 10.1073/pnas.73.3.945. View

Hang L, Theofilopoulos A, Dixon F . A spontaneous rheumatoid arthritis-like disease in MRL/l mice. J Exp Med. 1982; 155(6):1690-701. PMC: 2186697. DOI: 10.1084/jem.155.6.1690. View

Theofilopoulos A, Dixon F . Etiopathogenesis of murine SLE. Immunol Rev. 1981; 55:179-216. DOI: 10.1111/j.1600-065x.1981.tb00343.x. View

McDonald J, Levick J . Morphology of surface synoviocytes in situ at normal and raised joint pressure, studied by scanning electron microscopy. Ann Rheum Dis. 1988; 47(3):232-40. PMC: 1003490. DOI: 10.1136/ard.47.3.232. View

10.

Woolley D, Harris Jr E, Mainardi C, Brinckerhoff C . Collagenase immunolocalization in cultures of rheumatoid synovial cells. Science. 1978; 200(4343):773-5. DOI: 10.1126/science.205952. View

11.

OSullivan F, Fassbender H, Gay S, Koopman W . Etiopathogenesis of the rheumatoid arthritis-like disease in MRL/l mice. I. The histomorphologic basis of joint destruction. Arthritis Rheum. 1985; 28(5):529-36. DOI: 10.1002/art.1780280511. View

12.

Gay S, Gay R . Cellular basis and oncogene expression of rheumatoid joint destruction. Rheumatol Int. 1989; 9(3-5):105-13. DOI: 10.1007/BF00271866. View

13.

Edwards J, Cooke A, Moore A, Collins C, Hay F, Willoughby D . Connective tissue abnormalities in MRL/1 mice. Ann Rheum Dis. 1986; 45(6):512-8. PMC: 1001925. DOI: 10.1136/ard.45.6.512. View

14.

Leisen J, Duncan H, Riddle J, Pitchford W . The erosive front: a topographic study of the junction between the pannus and the subchondral plate in the macerated rheumatoid metacarpal head. J Rheumatol. 1988; 15(1):17-22. View

15.

Cylwik B, Jodczyk K, BERNACKA K, Duda D . [Scanning electron-microscopical studies of the articular surface of rats with adjuvant-induced arthritis]. Reumatologia. 1978; 16(2):217-24. View

16.

Woolley D, Brinckerhoff C, Mainardi C, Vater C, Evanson J, Harris Jr E . Collagenase production by rheumatoid synovial cells: morphological and immunohistochemical studies of the dendritic cell. Ann Rheum Dis. 1979; 38(3):262-70. PMC: 1000449. DOI: 10.1136/ard.38.3.262. View

17.

Garner D, McGillivray D . Living articular cartilage is not smooth. The structure of mammalian and avian joint surfaces demonstrated in vivo by immersion incident light microscopy. Ann Rheum Dis. 1971; 30(1):3-9. PMC: 1005718. DOI: 10.1136/ard.30.1.3. View

18.

Pataki A . Polyarthritis in MRL lpr/lpr mice. Rheumatol Int. 1985; 5(3):113-20. DOI: 10.1007/BF00541330. View