Alpha 2.0
Login Register
» Articles » PMID: 13972632

Studies on Immunity in Anthrax. X. Gel-adsorbed Protective Antigen for Immunization of Man

Overview
Journal J Bacteriol
Publisher American Society for Microbiology
Specialty Microbiology
Date 1963 Jan 1
PMID 13972632
Citations 21
Authors
M Puziss
G G Wright
Affiliations
Soon will be listed here.
Abstract

Puziss, Milton (Fort Detrick, Frederick, Md.) and George G. Wright. Studies on immunity in anthrax. X. Gel-adsorbed protective antigen for immunization of man. J. Bacteriol. 85:230-236. 1963.-Protective antigen in filtrates of anaerobic cultures of Bacillus anthracis was not readily precipitable by alum or other aluminum salts, but was adsorbed onto a preformed aluminum hydroxide gel under specific conditions. The adsorbed product was highly effective in immunizing rabbits, guinea pigs, and mice against challenge with virulent B. anthracis spores. Gel-adsorbed anaerobic antigens preserved with 1:10,000 thimerosal were unstable on storage at 4 C; replacement of this preservative with 1:40,000 benzethonium chloride produced a more stable product. Addition of 0.0009% formalin further increased the stability during accelerated aging at 37 C. The final product had high protective activity in animals and was well tolerated in man.

Citing Articles

A Historical Review of Military Medical Strategies for Fighting Infectious Diseases: From Battlefields to Global Health.

Biselli R, Nisini R, Lista F, Autore A, Lastilla M, De Lorenzo G Biomedicines. 2022; 10(8).

PMID: 36009598 PMC: 9405556. DOI: 10.3390/biomedicines10082050.

Intramuscular delivery of adenovirus serotype 5 vector expressing humanized protective antigen induces rapid protection against anthrax that may bypass intranasally originated preexisting adenovirus immunity.

Wu S, Zhang Z, Yu R, Zhang J, Liu Y, Song X Clin Vaccine Immunol. 2013; 21(2):156-64.

PMID: 24307239 PMC: 3910935. DOI: 10.1128/CVI.00560-13.

Stochastic humoral immunity to Bacillus anthracis protective antigen: identification of anti-peptide IgG correlating with seroconversion to Lethal Toxin neutralization.

Dumas E, Nguyen M, Cox P, Rodgers H, Peterson J, James J Vaccine. 2013; 31(14):1856-63.

PMID: 23415781 PMC: 3614092. DOI: 10.1016/j.vaccine.2013.01.040.

A Bacillus anthracis strain deleted for six proteases serves as an effective host for production of recombinant proteins.

Pomerantsev A, Pomerantseva O, Moayeri M, Fattah R, Tallant C, Leppla S Protein Expr Purif. 2011; 80(1):80-90.

PMID: 21827967 PMC: 3183367. DOI: 10.1016/j.pep.2011.05.016.

Integrating experimental (in vitro and in vivo) neurotoxicity studies of low-dose thimerosal relevant to vaccines.

Dorea J Neurochem Res. 2011; 36(6):927-38.

PMID: 21350943 DOI: 10.1007/s11064-011-0427-0.

References
1.
Puziss M, Wright G . Studies on immunity in anthrax. VII. Carbohydrate metabolism of Bacillus anthracis in relation to elaboration of protective antigen. J Bacteriol. 1959; 78(1):137-45. PMC: 290495. DOI: 10.1128/jb.78.1.137-145.1959. View
2.
Wright G, GREEN T, KANODE Jr R . Studies on immunity in anthrax. V. Immunizing activity of alum-precipitated protective antigen. J Immunol. 1954; 73(6):387-91. View
3.
Stanley J, Smith H . Purification of factor I and recognition of a third factor of the anthrax toxin. J Gen Microbiol. 1961; 26:49-63. DOI: 10.1099/00221287-26-1-49. View
4.
Wright G, DUFF J, FIOCK M, Devlin H, SODERSTROM R . Studies on immunity to toxins of Clostridium botulinum. V. Detoxification of purified type A and type B toxins, and the antigenicity of univalent and bivalent aluminium phosphate adsorbed toxoids. J Immunol. 1960; 84:384-9. View
5.
Wright G, Puziss M, Neely W . Studies on immunity in anthrax. IX. Effect of variations in cultural conditions on elaboration of protective antigen by strains of Bacillus anthracis. J Bacteriol. 1962; 83:515-22. PMC: 279305. DOI: 10.1128/jb.83.3.515-522.1962. View