A Semi-empirical Approach for the Simulation of Circular Dichroism Spectra of Gramicidin A in a Model Membrane

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 1992 Jul 1

PMID 1384735

Citations 2

Authors

M C Bano

L Braco

C Abad

Affiliations

Soon will be listed here.

Abstract

In an extension of our previous work (Bañó, M. C., Braco, L., and Abad, C. 1991. Biochemistry. 30:886-94), the kinetics of dissociation of gramicidin A double-stranded dimers into beta 6.3-helical monomers in small unilamellar vesicles prepared following different protocols, were investigated using in combination circular dichroism (CD) and high-performance liquid chromatography (HPLC). The analysis of the data from both techniques according to a two-component model strongly supports that any given CD pattern of gramicidin incorporated in the phospholipid bilayer can be deconvoluted essentially as a linear combination of the reference subspectra calculated for the double-stranded dimer and the helical monomer. An HPLC-based, semi-empirical approach is proposed for the simulation of gramicidin CD curves in the model membrane used, and it is shown that the congruence between theoretical and experimental spectra is very satisfactory.

Citing Articles

Wavelength-selective fluorescence as a novel tool to study organization and dynamics in complex biological systems.

Mukherjee S, Chattopadhyay A J Fluoresc. 2013; 5(3):237-46.

PMID: 24226791 DOI: 10.1007/BF00723895.

Thiazolidinedione insulin sensitizers alter lipid bilayer properties and voltage-dependent sodium channel function: implications for drug discovery.

Rusinova R, Herold K, Sanford R, Greathouse D, Hemmings Jr H, Andersen O J Gen Physiol. 2011; 138(2):249-70.

PMID: 21788612 PMC: 3149818. DOI: 10.1085/jgp.201010529.

References

Szabo G, Urry D . N-acetyl gramicidin: single-channel properties and implications for channel structure. Science. 1979; 203(4375):55-7. DOI: 10.1126/science.83000. View

Shire S, Holladay L, Rinderknecht E . Self-association of human and porcine relaxin as assessed by analytical ultracentrifugation and circular dichroism. Biochemistry. 1991; 30(31):7703-11. DOI: 10.1021/bi00245a006. View

Sawyer D, Williams L, Whaley W, Koeppe 2nd R, Andersen O . Gramicidins A, B, and C form structurally equivalent ion channels. Biophys J. 1990; 58(5):1207-12. PMC: 1281065. DOI: 10.1016/S0006-3495(90)82461-9. View

Kuwajima K, Garvey E, Finn B, Matthews C, Sugai S . Transient intermediates in the folding of dihydrofolate reductase as detected by far-ultraviolet circular dichroism spectroscopy. Biochemistry. 1991; 30(31):7693-703. DOI: 10.1021/bi00245a005. View

Perczel A, Hollosi M, Tusnady G, Fasman G . Convex constraint analysis: a natural deconvolution of circular dichroism curves of proteins. Protein Eng. 1991; 4(6):669-79. DOI: 10.1093/protein/4.6.669. View

Wallace B . Structure of gramicidin A. Biophys J. 1986; 49(1):295-306. PMC: 1329637. DOI: 10.1016/S0006-3495(86)83642-6. View

Braco L, Bano M, Chillaron F, Abad C . Conformational species of gramicidin A in non-polar solvent. A kinetic and thermodynamic treatment in the absence and presence of phosphatidylcholine as studied by high-performance liquid chromatography. Biophys Chem. 1986; 25(3):297-305. DOI: 10.1016/0301-4622(86)80022-9. View

Killian J, Prasad K, Hains D, Urry D . The membrane as an environment of minimal interconversion. A circular dichroism study on the solvent dependence of the conformational behavior of gramicidin in diacylphosphatidylcholine model membranes. Biochemistry. 1988; 27(13):4848-55. DOI: 10.1021/bi00413a040. View

LoGrasso P, Moll 3rd F, Cross T . Solvent history dependence of gramicidin A conformations in hydrated lipid bilayers. Biophys J. 1988; 54(2):259-67. PMC: 1330292. DOI: 10.1016/S0006-3495(88)82955-2. View

10.

Pancoska P, Keiderling T . Systematic comparison of statistical analyses of electronic and vibrational circular dichroism for secondary structure prediction of selected proteins. Biochemistry. 1991; 30(28):6885-95. DOI: 10.1021/bi00242a012. View

11.

Arseniev A, Barsukov I, Bystrov V, Lomize A, Ovchinnikov YuA . 1H-NMR study of gramicidin A transmembrane ion channel. Head-to-head right-handed, single-stranded helices. FEBS Lett. 1985; 186(2):168-74. DOI: 10.1016/0014-5793(85)80702-x. View

12.

Shungu D, Hinton J, Koeppe 2nd R, Millett F . Investigation of the interaction between thallous ions and gramicidin A in dimyristoylphosphatidylcholine vesicles: a thallium-205 NMR equilibrium study. Biochemistry. 1986; 25(20):6103-8. DOI: 10.1021/bi00368a040. View

13.

Huang H, Olah G . Uniformly oriented gramicidin channels embedded in thick monodomain lecithin multilayers. Biophys J. 1987; 51(6):989-92. PMC: 1330033. DOI: 10.1016/S0006-3495(87)83427-6. View

14.

Tournois H, Killian J, Urry D, Bokking O, de Gier J, de Kruijff B . Solvent determined conformation of gramicidin affects the ability of the peptide to induce hexagonal HII phase formation in dioleoylphosphatidylcholine model membranes. Biochim Biophys Acta. 1987; 905(1):222-6. DOI: 10.1016/0005-2736(87)90026-5. View

15.

Killian J, Urry D . Conformation of gramicidin in relation to its ability to form bilayers with lysophosphatidylcholine. Biochemistry. 1988; 27(19):7295-301. DOI: 10.1021/bi00419a018. View

16.

Van Mau N, Trudelle Y, Daumas P, Heitz F . Mixed monolayers of linear gramicidins and phospholipid. Surface pressure and surface potential studies. Biophys J. 1988; 54(3):563-7. PMC: 1330356. DOI: 10.1016/S0006-3495(88)82990-4. View

17.

Bano M, Braco L, Abad C . HPLC study on the 'history' dependence of gramicidin A conformation in phospholipid model membranes. FEBS Lett. 1989; 250(1):67-71. DOI: 10.1016/0014-5793(89)80686-6. View

18.

Johnson Jr W . Secondary structure of proteins through circular dichroism spectroscopy. Annu Rev Biophys Biophys Chem. 1988; 17:145-66. DOI: 10.1146/annurev.bb.17.060188.001045. View

19.

Yang J, Wu C, Martinez H . Calculation of protein conformation from circular dichroism. Methods Enzymol. 1986; 130:208-69. DOI: 10.1016/0076-6879(86)30013-2. View

20.

Urry D . The gramicidin A transmembrane channel: a proposed pi(L,D) helix. Proc Natl Acad Sci U S A. 1971; 68(3):672-6. PMC: 389014. DOI: 10.1073/pnas.68.3.672. View