Development of CDNA Probes for Typing Group A Bovine Rotaviruses on the Basis of VP4 Specificity

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1992 Oct 1

PMID 1383267

Citations 6

Authors

A V Parwani

B I Rosen

M A McCrae

L J Saif

Affiliations

Soon will be listed here.

Abstract

Dot and Northern (RNA) blot hybridization assays were developed for the P typing of group A bovine rotaviruses (BRV) by using cDNA probes prepared from gene segment 4. The probes were prepared by polymerase chain reaction amplification of hyperdivergent regions (nucleotides 211 to 686) of BRV strain UK, IND, NCDV, and Cr VP4 cDNA by using specific oligonucleotide primers. The probes were P type specific (VP4) and exhibited little or no cross-reactivity with double-stranded RNA from heterologous rotavirus P types. Our studies indicate that at least three P types, as defined by polymerase chain reaction-derived VP4 gene probes from the UK, NCDV, and Cr strains, exist among the seven BRV isolates tested.

Citing Articles

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Characterization of field strains of group A bovine rotaviruses by using polymerase chain reaction-generated G and P type-specific cDNA probes.

Parwani A, Hussein H, Rosen B, Lucchelli A, Navarro L, Saif L J Clin Microbiol. 1993; 31(8):2010-5.

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Relative frequencies of G (VP7) and P (VP4) serotypes determined by polymerase chain reaction assays among Japanese bovine rotaviruses isolated in cell culture.

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Bovine rotavirus V1005 a P5, not a P12, type like all viruses in a German survey.

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