Proliferating Cell Nuclear Antigen Immunohistochemistry in Rat Aorta After Balloon Denudation. Comparison with Thymidine and Bromodeoxyuridine Labeling

Overview

Journal Am J Pathol

Publisher Elsevier

Specialty Pathology

Date 1992 Sep 1

PMID 1381560

Citations 12

Authors

U Zeymer

M C Fishbein

J S Forrester

B Cercek

Affiliations

Soon will be listed here.

Abstract

The authors compared detection of proliferating vascular smooth muscle cells (SMC) in vitro and in vivo with proliferating cell nuclear antigen (PCNA) immunohistochemistry and two established methods: [3H]thymidine autoradiography and bromodeoxyuridine immunohistochemistry. Labeling with [3H]thymidine and bromodeoxyuridine of rat vascular SMC in culture stained 11% +/- 2% and 11% +/- 1% of cells, and PCNA immunohistochemistry 22% +/- 2% of cells. Proliferation in the media of the denuded rat aortae was highest 3 days after denudation: 4.2%, 3.8%, and 4.7% of labeled cells with [3H]thymidine, bromodeoxyuridine, and PCNA, respectively. In the intima, proliferation was highest 7 days after denudation with 42% [3H]thymidine, 40% bromodeoxyuridine, and 46% PCNA-positive cells. With double labeling, all [3H]thymidine-positive cells were PCNA positive, whereas some cells were only positive for PCNA. The authors conclude that PCNA immunohistochemistry compares favorably with [3H]thymidine autoradiography, and bromodeoxyuridine immunohistochemistry.

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References

Coltrera M, Gown A . PCNA/cyclin expression and BrdU uptake define different subpopulations in different cell lines. J Histochem Cytochem. 1991; 39(1):23-30. DOI: 10.1177/39.1.1670579. View

Clowes A, Clowes M . Kinetics of cellular proliferation after arterial injury. II. Inhibition of smooth muscle growth by heparin. Lab Invest. 1985; 52(6):611-6. View

Lombardi D, Reidy M, Schwartz S . Methodologic considerations important in the accurate quantitation of aortic smooth muscle cell replication in the normal rat. Am J Pathol. 1991; 138(2):441-6. PMC: 1886205. View

Cercek B, Fishbein M, Forrester J, Helfant R, Fagin J . Induction of insulin-like growth factor I messenger RNA in rat aorta after balloon denudation. Circ Res. 1990; 66(6):1755-60. DOI: 10.1161/01.res.66.6.1755. View

Thaete L, Ahnen D, Malkinson A . Proliferating cell nuclear antigen (PCNA/cyclin) immunocytochemistry as a labeling index in mouse lung tissues. Cell Tissue Res. 1989; 256(1):167-73. DOI: 10.1007/BF00224731. View

Bravo R . Existence of two populations of cyclin/proliferating cell nuclear antigen during the cell cycle: association with DNA replication sites. J Cell Biol. 1987; 105(4):1549-54. PMC: 2114668. DOI: 10.1083/jcb.105.4.1549. View

Jaskulski D, Gatti C, Travali S, Calabretta B, Baserga R . Regulation of the proliferating cell nuclear antigen cyclin and thymidine kinase mRNA levels by growth factors. J Biol Chem. 1988; 263(21):10175-9. View

Gratzner H . Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication. Science. 1982; 218(4571):474-5. DOI: 10.1126/science.7123245. View

Van Dierendonck J, Wijsman J, Keijzer R, van de Velde C, Cornelisse C . Cell-cycle-related staining patterns of anti-proliferating cell nuclear antigen monoclonal antibodies. Comparison with BrdUrd labeling and Ki-67 staining. Am J Pathol. 1991; 138(5):1165-72. PMC: 1886031. View

10.

Gordon D, Reidy M, BENDITT E, Schwartz S . Cell proliferation in human coronary arteries. Proc Natl Acad Sci U S A. 1990; 87(12):4600-4. PMC: 54164. DOI: 10.1073/pnas.87.12.4600. View

11.

Hall P, Levison D, Woods A, Yu C, Kellock D, Watkins J . Proliferating cell nuclear antigen (PCNA) immunolocalization in paraffin sections: an index of cell proliferation with evidence of deregulated expression in some neoplasms. J Pathol. 1990; 162(4):285-94. DOI: 10.1002/path.1711620403. View

12.

Hall P, Levison D . Review: assessment of cell proliferation in histological material. J Clin Pathol. 1990; 43(3):184-92. PMC: 502326. DOI: 10.1136/jcp.43.3.184. View

13.

Travali S, Ku D, Rizzo M, Ottavio L, Baserga R, Calabretta B . Structure of the human gene for the proliferating cell nuclear antigen. J Biol Chem. 1989; 264(13):7466-72. View

14.

Galand P, Degraef C . Cyclin/PCNA immunostaining as an alternative to tritiated thymidine pulse labelling for marking S phase cells in paraffin sections from animal and human tissues. Cell Tissue Kinet. 1989; 22(5):383-92. DOI: 10.1111/j.1365-2184.1989.tb00223.x. View

15.

Bravo R, Frank R, Blundell P . Cyclin/PCNA is the auxiliary protein of DNA polymerase-delta. Nature. 1987; 326(6112):515-7. DOI: 10.1038/326515a0. View

16.

Kurki P, Ogata K, Tan E . Monoclonal antibodies to proliferating cell nuclear antigen (PCNA)/cyclin as probes for proliferating cells by immunofluorescence microscopy and flow cytometry. J Immunol Methods. 1988; 109(1):49-59. DOI: 10.1016/0022-1759(88)90441-3. View

17.

Owens G, Loeb A, Gordon D, Thompson M . Expression of smooth muscle-specific alpha-isoactin in cultured vascular smooth muscle cells: relationship between growth and cytodifferentiation. J Cell Biol. 1986; 102(2):343-52. PMC: 2114077. DOI: 10.1083/jcb.102.2.343. View

18.

Waseem N, Lane D . Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form. J Cell Sci. 1990; 96 ( Pt 1):121-9. DOI: 10.1242/jcs.96.1.121. View