Validation of a Simple Radiochemical Assay Measuring Hydrolysis of Choline-labelled Microsomal Phosphatidylcholine by Phospholipase C. PH-dependence

Overview

Journal Biochem J

Specialty Biochemistry

Date 1976 Dec 15

PMID 13785

Citations 1

Authors

B R. Cater

P Trivedi

T Hallinan

Affiliations

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Abstract

Selective hydrolysis of phosphatidylcholine species, which are selectively radioactively labelled in vivo, does not appear to interfere with a radiochemical assay for hydrolysis of microsomal phosphatidylcholine by C-type phospholipases from Bacillus cereus or Clostridium perfringens. Both phospholipases substantially hydrolysed phosphatidylcholine over the pH range 4.0-10.0.

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PMID: 96816 PMC: 1184033. DOI: 10.1042/bj1710821.

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