The Direct Isolation of the Mitotic Apparatus

Overview

Journal J Biophys Biochem Cytol

Specialty Cell Biology

Date 1961 Aug 1

PMID 13768661

Citations 24

Authors

D MAZIA

J M Mitchison

H Medina

P Harris

Affiliations

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Abstract

A method for isolating the mitotic apparatus from dividing sea urchin eggs without the use of ethyl alcohol or of detergents is described. In the present method, the eggs are dispersed directly in a medium containing 1 M (to 1.15 M) sucrose, 0.15 M dithiodiglycol, and 0.001 M Versene at pH 6, releasing the visibly intact mitotic apparatus. The method is designed for studies of enzyme activities, lipid components, and the variables affecting the stability of the apparatus.

Citing Articles

A Brief History of Research on Mitotic Mechanisms.

McIntosh J, Hays T Biology (Basel). 2016; 5(4).

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THE ANTICOAGULANT AND ANTILYMPHOMA PROPERTIES OF ARSENIC AZOPROTEINS : II. COMBINATION OF ARSENIC AZOPROTEINS WITH FIBRINOGEN AND POLYMERIZING FIBRIN IN VITRO: ALTERATIONS IN MITOSIS OF LYMPHOMA 6C3HED CELLS INDUCED IN VIVO WITH ARSENIC AZOPROTEINS:....

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Cell division screens and dynamin.

Bonner M, Skop A Biochem Soc Trans. 2008; 36(Pt 3):431-5.

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THE MITOTIC APPARATUS. PHYSICAL-CHEMICAL FACTORS CONTROLLING STABILITY.

Kane R J Cell Biol. 1965; 25:SUPPL:137-44.

PMID: 14342827 PMC: 2106617. DOI: 10.1083/jcb.25.1.137.

ISOLATION OF SPINDLES FROM THE SURF CLAM SPISULA SOLIDISSIMA.

REBHUN L, SHARPLESS T J Cell Biol. 1964; 22:488-91.

PMID: 14203394 PMC: 2106450. DOI: 10.1083/jcb.22.2.488.

References

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