Stable and Functional Expression of the Calcium Channel Alpha 1 Subunit from Smooth Muscle in Somatic Cell Lines

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1992 Jun 1

PMID 1376244

Citations 17

Authors

E Bosse

R Bottlender

T Kleppisch

J Hescheler

A Welling

F Hofmann

V Flockerzi

Affiliations

Soon will be listed here.

Abstract

Voltage-activated calcium channels are membrane spanning proteins that allow the controlled entry of Ca2+ into the cytoplasm of cells. The principal channel forming subunit of an L-type calcium channel is the alpha 1 subunit. Transfection of Chinese hamster ovary (CHO) cells with complementary DNA encoding the calcium channel alpha 1 subunit from smooth muscle led to the expression of functional calcium channels which bind calcium channel blockers and show the voltage-dependent activation and slow inactivation and unitary current conductance characteristic of calcium channels in smooth muscle. The currents mediated by these channels are sensitive towards dihydropyridine-type blockers and agonists indicating that the calcium channel blocker receptor sites were present in functional form. The smooth muscle alpha 1 subunit cDNA alone is sufficient for stable expression of functional calcium channels with the expected kinetic and pharmacological properties in mammalian somatic cells.

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