Balbiani Ring HnRNP Substructure Visualized by Selective Staining and Electron Spectroscopic Imaging

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1992 May 1

PMID 1374066

Citations 5

Authors

A L Olins

D E Olins

D P Bazett-Jones

Affiliations

Soon will be listed here.

Abstract

The Balbiani Rings (BR) in the polytene chromosomes of Chironomus salivary glands are intense sites of transcription. The nascent RNPs fold during transcription into 40-50-nm granules, containing in the mature transcript approximately 37-kb RNA. Using a new nucleic acid specific stain, osmium ammine B on Lowicryl sections, in combination with electron energy filtered imaging of sections containing BR granules, we demonstrate a RNA-rich particulate substructure (10-nm particle diameter; 10-12 particles per BR granule). Elemental imaging supports that these particles are enriched in phosphorus. The possible relationship of these RNA-rich particles to ribonucleosomes is discussed, as well as models for their arrangement in the mature BR granules.

Citing Articles

Low-voltage scanning electron microscopy study of lampbrush chromosomes and nuclear bodies in avian and amphibian oocytes.

Kulikova T, Khodyuchenko T, Petrov Y, Krasikova A Sci Rep. 2016; 6:36878.

PMID: 27857188 PMC: 5114574. DOI: 10.1038/srep36878.

Electron spectroscopic imaging analyses of the distribution of phosphorus in Balbiani ring granules and in the surrounding nucleoplasm.

Vazquez-Nin G, Echeverria O, von Schack M, Fakan S Chromosoma. 1997; 105(6):360-8.

PMID: 9087378 DOI: 10.1007/BF02529751.

Electron microscope tomography of Balbiani Ring hnRNP substructure.

Olins A, Olins D, LEVY H, Shah M, Bazett-Jones D Chromosoma. 1993; 102(2):137-44.

PMID: 8432195 DOI: 10.1007/BF00356031.

A new type of ribonucleoprotein constituent of the polytene nucleus of the salivary glands of Chironomus thummi and Ch. tentans.

Vazquez-Nin G, Echeverria O, Fakan S Chromosoma. 1993; 102(10):693-9.

PMID: 8149810 DOI: 10.1007/BF00650895.

Modeling the 3-D RNA distribution in the Balbiani ring granule.

Olins A, Olins D, Olman V, LEVY H, Bazett-Jones D Chromosoma. 1994; 103(5):302-10.

PMID: 7821085 DOI: 10.1007/BF00417877.

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