Tracking Genetically Engineered Bacteria: Monoclonal Antibodies Against Surface Determinants of the Soil Bacterium Pseudomonas Putida 2440

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1992 May 1

PMID 1373718

Citations 8

Authors

M I Ramos-Gonzalez

F Ruiz-Cabello

I Brettar

F Garrido

J L Ramos

Affiliations

Soon will be listed here.

Abstract

Assessment of potential risks involved in the release of genetically engineered microorganisms is facilitated by the availability of monoclonal antibodies (MAbs), a tool potentially able to monitor specific organisms. We raised a bank of MAbs against the soil bacterium Pseudomonas putida 2440, which is a host for modified TOL plasmids and other recombinant plasmids. Three MAbs, 7.3B, 7.4D, and 7.5D, were highly specific and recognized only P. putida bacteria. Furthermore, we developed a semiquantitative dot blot assay that allowed us to detect as few as 100 cells per spot. A 40-kDa cell surface protein was the target for MAbs 7.4D and 7.5D. Detection of the cell antigen depended on the bacterial growth phase and culture medium. The O antigen of lipopolysaccharide seems to be the target for MAb 7.3B, and its in vivo detection was independent of the bacterial growth phase and culture medium. MAb 7.3B was used successfully to track P. putida (pWW0) released in unsterile lake mesocosms.

Citing Articles

A safety type of genetically engineered bacterium that degrades chemical pesticides.

Li Q, Li J, Kang K, Wu Y AMB Express. 2020; 10(1):33.

PMID: 32072335 PMC: 7028883. DOI: 10.1186/s13568-020-00967-y.

Fate of Pseudomonas putida after release into lake water mesocosms: Different survival mechanisms in response to environmental conditions.

Brettar I, Ramos-Gonzalez M, Ramos J, Hofle M Microb Ecol. 2013; 27(2):99-122.

PMID: 24190270 DOI: 10.1007/BF00165812.

Tracking the Response of Burkholderia cepacia G4 5223-PR1 in Aquifer Microcosms.

Winkler J, Timmis K, Snyder R Appl Environ Microbiol. 1995; 61(2):448-55.

PMID: 16534928 PMC: 1388346. DOI: 10.1128/aem.61.2.448-455.1995.

Culturability and Expression of Outer Membrane Proteins during Carbon, Nitrogen, or Phosphorus Starvation of Pseudomonas fluorescens DF57 and Pseudomonas putida DF14.

Kragelund L, Nybroe O Appl Environ Microbiol. 1994; 60(8):2944-8.

PMID: 16349359 PMC: 201747. DOI: 10.1128/aem.60.8.2944-2948.1994.

Involvement of the cis/trans isomerase Cti in solvent resistance of Pseudomonas putida DOT-T1E.

Junker F, Ramos J J Bacteriol. 1999; 181(18):5693-700.

PMID: 10482510 PMC: 94089. DOI: 10.1128/JB.181.18.5693-5700.1999.

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