Longitudinal Study of Plasmodium Falciparum Polymorphic Antigens in a Malaria-endemic Population

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1992 Mar 1

PMID 1371764

Citations 24

Authors

D J Conway

B M Greenwood

J S McBride

Affiliations

Soon will be listed here.

Abstract

Plasmodium falciparum merozoite surface antigens MSP1 and MSP2 and an exported antigen, Exp-1, exhibit allelic polymorphism in natural populations. To explain this, one hypothesis is that antigen polymorphisms are maintained by frequency-dependent immune selection. An expectation of the hypothesis is that rare variants have an advantage over common variants because of a lower level of acquired immunity against them and thus increase in frequency until an equilibrium is attained. To test this hypothesis, the frequencies of polymorphic epitopes of MSP1, MSP2, and Exp-1 were determined among isolates from malaria patients in an urban area of The Gambia, during different periods of one transmission season (1988) and in different years (1982, 1983, 1988, and 1989). The frequencies remained very stable throughout the period of study, alternative epitope variants remaining either rare or common, without shifts in relative frequencies. These results are discussed with reference to the immune-selection hypothesis, with the conclusion that frequencies of the major dimorphic serological classes of MSP1 are probably not maintained by immune selection.

Citing Articles

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Multifunctional IgG/IgM antibodies and cellular cytotoxicity are elicited by the full-length MSP1 SumayaVac-1 malaria vaccine.

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References

McBride J, Newbold C, Anand R . Polymorphism of a high molecular weight schizont antigen of the human malaria parasite Plasmodium falciparum. J Exp Med. 1985; 161(1):160-80. PMC: 2187544. DOI: 10.1084/jem.161.1.160. View

BRAY R, GUNDERS A, Burgess R, Freeman J, ETZEL E, GUTTUSO C . The inoculation of semi-immune Africans with sporozoites of Laverania falcipara (Plasmodium falciparum) in Liberia. Riv Malariol. 1962; 41:199-210. View

Tanabe K, Mackay M, Goman M, Scaife J . Allelic dimorphism in a surface antigen gene of the malaria parasite Plasmodium falciparum. J Mol Biol. 1987; 195(2):273-87. DOI: 10.1016/0022-2836(87)90649-8. View

Stanley H, Howard R, REESE R . Recognition of a Mr 56K glycoprotein on the surface of Plasmodium falciparum merozoites by mouse monoclonal antibodies. J Immunol. 1985; 134(5):3439-44. View

Wilson R, McGregor I, Hall P, Williams K, Bartholomew R . Antigens associated with Plasmodium falciparum infections in man. Lancet. 1969; 2(7613):201-5. DOI: 10.1016/s0140-6736(69)91437-8. View

Carter R, McGregor I . Enzyme variation in Plasmodium falciparum in the Gambia. Trans R Soc Trop Med Hyg. 1973; 67(6):830-7. DOI: 10.1016/0035-9203(73)90011-4. View

Hall R, Hyde J, Goman M, Simmons D, Hope I, Mackay M . Major surface antigen gene of a human malaria parasite cloned and expressed in bacteria. Nature. 1984; 311(5984):379-82. DOI: 10.1038/311379a0. View

Hommel M, David P, Oligino L . Surface alterations of erythrocytes in Plasmodium falciparum malaria. Antigenic variation, antigenic diversity, and the role of the spleen. J Exp Med. 1983; 157(4):1137-48. PMC: 2186973. DOI: 10.1084/jem.157.4.1137. View

Perrin L, Merkli B, Loche M, Chizzolini C, Smart J, RICHLE R . Antimalarial immunity in Saimiri monkeys. Immunization with surface components of asexual blood stages. J Exp Med. 1984; 160(2):441-51. PMC: 2187457. DOI: 10.1084/jem.160.2.441. View

10.

TRAGER W, Jensen J . Human malaria parasites in continuous culture. Science. 1976; 193(4254):673-5. DOI: 10.1126/science.781840. View

11.

Conway D, Greenwood B, McBride J . The epidemiology of multiple-clone Plasmodium falciparum infections in Gambian patients. Parasitology. 1991; 103 Pt 1:1-6. DOI: 10.1017/s0031182000059217. View

12.

Conway D, McBride J . Population genetics of Plasmodium falciparum within a malaria hyperendemic area. Parasitology. 1991; 103 Pt 1:7-16. DOI: 10.1017/s0031182000059229. View

13.

Conway D, Rosario V, Oduola A, Salako L, Greenwood B, McBride J . Plasmodium falciparum: intragenic recombination and nonrandom associations between polymorphic domains of the precursor to the major merozoite surface antigens. Exp Parasitol. 1991; 73(4):469-80. DOI: 10.1016/0014-4894(91)90071-4. View

14.

Greenwood B, Armstrong J . Comparison of two simple methods for determining malaria parasite density. Trans R Soc Trop Med Hyg. 1991; 85(2):186-8. DOI: 10.1016/0035-9203(91)90015-q. View

15.

Fenton B, Clark J, Khan C, Robinson J, Walliker D, Ridley R . Structural and antigenic polymorphism of the 35- to 48-kilodalton merozoite surface antigen (MSA-2) of the malaria parasite Plasmodium falciparum. Mol Cell Biol. 1991; 11(2):963-71. PMC: 359759. DOI: 10.1128/mcb.11.2.963-971.1991. View

16.

Fruh K, Doumbo O, Muller H, Koita O, McBride J, Crisanti A . Human antibody response to the major merozoite surface antigen of Plasmodium falciparum is strain specific and short-lived. Infect Immun. 1991; 59(4):1319-24. PMC: 257845. DOI: 10.1128/iai.59.4.1319-1324.1991. View

17.

Thomas A, Carr D, Carter J, Lyon J . Sequence comparison of allelic forms of the Plasmodium falciparum merozoite surface antigen MSA2. Mol Biochem Parasitol. 1990; 43(2):211-20. DOI: 10.1016/0166-6851(90)90146-d. View

18.

Smythe J, Peterson M, Coppel R, Saul A, Kemp D, Anders R . Structural diversity in the 45-kilodalton merozoite surface antigen of Plasmodium falciparum. Mol Biochem Parasitol. 1990; 39(2):227-34. DOI: 10.1016/0166-6851(90)90061-p. View

19.

Howard R, Stanley H, Campbell G, Langreth S, REESE R . Two Plasmodium falciparum merozoite surface polypeptides share epitopes with a single Mr 185 000 parasite glycoprotein. Mol Biochem Parasitol. 1985; 17(1):61-77. DOI: 10.1016/0166-6851(85)90128-8. View

20.

Marsh K, Howard R . Antigens induced on erythrocytes by P. falciparum: expression of diverse and conserved determinants. Science. 1986; 231(4734):150-3. DOI: 10.1126/science.2417315. View