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Regulation of Phosphate Metabolism in Neurospora Crassa: Identification of the Structural Gene for Repressible Acid Phosphatase

Overview
Journal Genetics
Publisher Oxford University Press
Specialty Genetics
Date 1976 Oct 1
PMID 137163
Citations 12
Authors
R E Nelson
J F Lehman
R L Metzenberg
Affiliations
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Abstract

A mutant of Neurospora crassa with an altered repressible acid phosphatase has been isolated. The enzyme is much more thermolabile than that of wild type, and has an increased Michaelis constant. Tests of allelic interactions (in partial diploids) and in vitro mixing experiments were consistent with the mutation being in the structural gene for the enzyme. This gene, pho-3, was found to be located in the right arm of Linkage Group IV (LGIV). Thus, pho-3 and the structural gene for repressible alkaline phosphatase, pho-2 (LG V), map in separate linkage groups and cannot be part of the same operon. Neither of these structural genes is linked to the known regulatory genes, nuc-1 (LG I), nuc-2 (LG II), and preg (LG II).

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References
1.
Gleason M, Metzenberg R . Regulation of phosphate metabolism in Neurospora crassa: isolation of mutants deficient in ther repressible alkaline phosphatase. Genetics. 1974; 78(2):645-59. PMC: 1213225. DOI: 10.1093/genetics/78.2.645. View
2.
Jacobs M, NYC J, Brown D . Isolation and chemical properties of a repressible acid phosphatase in Neurospora crassa. J Biol Chem. 1971; 246(5):1419-25. View
3.
Lowendorf H, SLAYMAN C . Genetic regulation of phosphate transport system II in Neurospora. Biochim Biophys Acta. 1975; 413(1):95-103. DOI: 10.1016/0005-2736(75)90061-9. View
4.
LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View
5.
Hasunuma K . Repressible extracellular nucleases in Neurospora crassa. Biochim Biophys Acta. 1973; 319(3):288-93. DOI: 10.1016/0005-2787(73)90168-8. View