Anti-platelet Autoantibodies from ITP Patients Recognize an Epitope in GPIIb/IIIa Deduced by Complementary Hydropathy

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1992 Jan 1

PMID 1371492

Citations 1

Authors

S J de Souza

J Sabbaga

E DAmico

R Pasqualini

R BRENTANI

Affiliations

Soon will be listed here.

Abstract

Idiopathic thrombocytopenic purpura (ITP) is a frequent platelet disorder due to the presence of anti-platelet autoantibodies. Recently a fibronectin/fibrinogen receptor in platelets, integrin GPIIb/IIIa, has been implicated as the antigen in chronic ITP. To examine the epitopes involved in the autoimmune response against GPIIb/IIIa we have used concepts from the complementary hydropathy principle. We used the peptide Trp-Thr-Val-Pro-Thr-Ala, WTVPTA (deduced from the complementary nucleotide sequence to that which codes for the Arg-Gly-Asp, RGD, domain in fibronectin), to test the immunologic activity of ITP sera. Sera from 31 patients with clinically defined ITP were tested in ELISA for reactivity towards WTVPTA and affinity purified GPIIb/IIIa. Seventeen sera (57%) reacted strongly with the glycoprotein complex, five of which reacted with the peptide. By affinity chromatography of one of these sera, we were able to show that antibodies that bind to the peptide are within the population that binds to GPIIb/IIIa. Liquid phase competition experiments revealed that binding of ITP serum to WTVPTA was inhibited only by a hydropathically compatible peptide. Our data indicate that autoantibodies can bind to hydropathically generated antigenic determinants and thus, render these peptides clinically important as diagnostic tools.

Citing Articles

Comparative study of IgG binding to megakaryocytes in immune and myelodysplastic thrombocytopenic patients.

Elzaeem D, Sharkawi E, Zaki E, Ghobrial A, El-Fatah A, El-Hamed W Ann Hematol. 2021; 100(7):1701-1709.

PMID: 33982136 DOI: 10.1007/s00277-021-04556-2.

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