Atomic Structure of Single-stranded DNA Bacteriophage Phi X174 and Its Functional Implications

Overview

Journal Nature

Specialty Science

Date 1992 Jan 9

PMID 1370343

Citations 89

Authors

R McKenna

D Xia

P Willingmann

L L Ilag

S Krishnaswamy

M G Rossmann

N H Olson

T S Baker

N L INCARDONA

Affiliations

Soon will be listed here.

Abstract

The mechanism of DNA ejection, viral assembly and evolution are related to the structure of bacteriophage phi X174. The F protein forms a T = 1 capsid whose major folding motif is the eight-stranded antiparallel beta barrel found in many other icosahedral viruses. Groups of 5 G proteins form 12 dominating spikes that enclose a hydrophilic channel containing some diffuse electron density. Each G protein is a tight beta barrel with its strands running radially outwards and with a topology similar to that of the F protein. The 12 'pilot' H proteins per virion may be partially located in the putative ion channel. The small, basic J protein is associated with the DNA and is situated in an interior cleft of the F protein. Tentatively, there are three regions of partially ordered DNA structure,

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