Alpha 2.0
Login Register
» Articles » PMID: 1367899

Metabolic Roles of Peptone and Yeast Extract for the Culture of a Recombinant Strain of Escherichia Coli

Overview
Journal J Ind Microbiol
Date 1991 Oct 1
PMID 1367899
Citations 15
Authors
N Nancib
C Branlant
J Boudrant
Affiliations
Soon will be listed here.
Abstract

The influence of complex compounds on the growth of a recombinant strain of Escherichia coli containing the gene encoding glyceraldehyde 3-phosphate dehydrogenase, as well as the production of this enzyme have been studied. Batchwise cultures led to an accumulation of acetate, which was not utilized in a yeast extract-free medium. After glucose exhaustion, growth stopped and enzyme activity decreased. Whereas yeast extract allowed acetate assimilation and growth, peptone stabilized the enzymatic activity. The addition of both compounds resulted in optimal performances for enzyme production.

Citing Articles

Ecuadorian Cacao Mucilage as a Novel Culture Medium Ingredient: Unveiling Its Potential for Microbial Growth and Biotechnological Applications.

Guzman-Armenteros T, Guerra L, Ruales J, Ramos-Guerrero L Foods. 2025; 14(2).

PMID: 39856926 PMC: 11764727. DOI: 10.3390/foods14020261.

Substitute Yeast Extract While Maintaining Performance: Showcase Amorpha-4,11-Diene Production.

Castillo-Saldarriaga C, Santos C, Sarria S, Ajikumar P, Takors R Microb Biotechnol. 2024; 17(11):e70056.

PMID: 39570580 PMC: 11580704. DOI: 10.1111/1751-7915.70056.

System analysis of Lipomyces starkeyi during growth on various plant-based sugars.

Deewan A, Liu J, Jagtap S, Yun E, Walukiewicz H, Jin Y Appl Microbiol Biotechnol. 2022; 106(17):5629-5642.

PMID: 35906440 DOI: 10.1007/s00253-022-12084-w.

Intermittent induction of LEA peptide by lactose enhances the expression of insecticidal proteins in Bacillus thuringiensis.

Akthar M, Shimokawa T, Wu Y, Arita T, Mizuta K, Isono Y FEBS Open Bio. 2022; 12(8):1534-1541.

PMID: 35638574 PMC: 9340782. DOI: 10.1002/2211-5463.13448.

Optimization of Multiparameters for Increased Yields of Cytochrome B5 in Bioreactors.

Pereira R, de Carvalho C Molecules. 2021; 26(14).

PMID: 34299423 PMC: 8306036. DOI: 10.3390/molecules26144148.

References
1.
Branlant G, Flesch G, Branlant C . Molecular cloning of the glyceraldehyde-3-phosphate dehydrogenase genes of Bacillus stearothermophilus and Escherichia coli, and their expression in Escherichia coli. Gene. 1983; 25(1):1-7. DOI: 10.1016/0378-1119(83)90161-0. View
2.
Clark D . The fermentation pathways of Escherichia coli. FEMS Microbiol Rev. 1989; 5(3):223-34. DOI: 10.1016/0168-6445(89)90033-8. View
3.
HOLMS W, Bennett P . Regulation of isocitrate dehydrogenase activity in Escherichia coli on adaptation to acetate. J Gen Microbiol. 1971; 65(1):57-68. DOI: 10.1099/00221287-65-1-57. View
4.
Mougin A, Corbier C, Soukri A, Wonacott A, Branlant C, Branlant G . Use of site-directed mutagenesis to probe the role of Cys149 in the formation of charge-transfer transition in glyceraldehyde-3-phosphate dehydrogenase. Protein Eng. 1988; 2(1):45-8. DOI: 10.1093/protein/2.1.45. View
5.
Li X, Robbins Jr J, Taylor K . The production of recombinant beta-galactosidase in Escherichia coli in yeast extract enriched medium. J Ind Microbiol. 1990; 5(2-3):85-93. DOI: 10.1007/BF01573857. View