Spontaneous and PHA-induced Rosetting of Human Blood, Tonsil Lymphocytes and MLC Blasts with Sheep, Human and Horse Erythrocytes

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1976 Jul 1

PMID 136324

Citations 5

Authors

P Gergely

F Vanky

E Klein

Affiliations

Soon will be listed here.

Abstract

Compared to peripheral blood lymphocytes the ability of human tonsil T cells and MLC blasts to bind sheep, human and horse erythrocytes was found to be increased. Tonsil and MLC T cells were able to bind sheep red blood cells without any cold incubation, i.e. they were 'early' rosettes, and higher percentage of human and horse erythrocyte rosettes were formed by these cells. Low doses of phytohaemagglutinin increased the proportion of rosettes between peripheral blood, tonsil, MLC cells and human and horse erythrocytes. PHA acted only on T cells, and not on B cells, lymphoblastoid B and other cell lines. On the ground of the stronger rosetting property of MLC blasts and tonsil cells, it is likely that the T cells responsible for binding of horse and human erythrocytes after PHA treatment are 'early' or 'active' rosetting cells.

Citing Articles

Depressed lectin-dependent cell-mediated cytotoxicity against adherent HEP-2 cells in patients with carcinoma of the uterine cervix.

Perl A, Lang I, SOMOS P, Gergely P Cancer Immunol Immunother. 1983; 15(2):155-8.

PMID: 6553513 PMC: 11039231. DOI: 10.1007/BF00199708.

Study of human T and B lymphocytes with heterologous antisera. III. Immunofluorescence studies on tonsil sections.

LAMELIN J, Thomasset N, Andre C, BROCHIER J, Revillard J Immunology. 1978; 35(3):463-9.

PMID: 359461 PMC: 1457641.

In vitro effect of transfer factor on active rosettes and leucocyte migration of patients with cancer.

Nekam K, Kalmar L, Gergely P, Kelemen G, Fekete B, Lang I Clin Exp Immunol. 1977; 27(3):416-20.

PMID: 324669 PMC: 1540949.

Clinical immunological study of T lymphocytes and EA rosette forming cells in tonsils.

Gyeney L, Meretey K, Falus A, Bohm U, Bozsoky S Arch Otorhinolaryngol. 1978; 221(2):105-13.

PMID: 312640 DOI: 10.1007/BF00455881.

Blastogenic response of purified human T-lymphocyte populations to Epstein-Barr virus (EBV).

Gergely P, Ernberg I, Klein G, Steinitz M Clin Exp Immunol. 1977; 30(3):347-53.

PMID: 203426 PMC: 1541156.

References

Yu D . Human lymphocyte subpopulation: early and late rosettes. J Immunol. 1975; 115(1):91-3. View

Jondal M, Klein E, Yefenof E . Surface markers on human B and T lymphocytes. VII. Rosette formation between peripheral T lymphocytes and lymphoblastoid B-cell lines. Scand J Immunol. 1975; 4(3):259-66. DOI: 10.1111/j.1365-3083.1975.tb02625.x. View

FOLEY G, Lazarus H, Farber S, Uzman B, Boone B, McCarthy R . CONTINUOUS CULTURE OF HUMAN LYMPHOBLASTS FROM PERIPHERAL BLOOD OF A CHILD WITH ACUTE LEUKEMIA. Cancer. 1965; 18:522-9. DOI: 10.1002/1097-0142(196504)18:4<522::aid-cncr2820180418>3.0.co;2-j. View

PULVERTAFT J . A STUDY OF MALIGNANT TUMOURS IN NIGERIA BY SHORT-TERM TISSUE CULTURE. J Clin Pathol. 1965; 18:261-73. PMC: 472922. DOI: 10.1136/jcp.18.3.261. View

Glasgow A, Polgar P, Saporoschetz I, Kim H, RUTENBURG A, Mannick J . Phytohemagglutinin stimulation of human lymphocytes. Failure to detect an early increase in cyclic AMP concentration. Clin Immunol Immunopathol. 1975; 3(3):353-62. DOI: 10.1016/0090-1229(75)90022-7. View

Dodd N . Letter: PHA and lymphocyte membrane fluidity. Nature. 1975; 257(5529):827-8. DOI: 10.1038/257827d0. View

Pellegrino M, Ferrone S, Dierich M, Reisfeld R . Enhancement of sheep red blood cell human lymphocyte rosette formation by the sulfhydryl compound 2-amino ethylisothiouronium bromide. Clin Immunol Immunopathol. 1975; 3(3):324-33. DOI: 10.1016/0090-1229(75)90019-7. View

Tak Yan Yu D . Lymphocyte subpopulations. Human red blood cell rosettes. Clin Exp Immunol. 1975; 20(2):311-22. PMC: 1538188. View

Jondal M, Holm G, Wigzell H . Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972; 136(2):207-15. PMC: 2139198. DOI: 10.1084/jem.136.2.207. View

10.

Wybran J, Carr M, Fudenberg H . The human rosette-forming cell as a marker of a population of thymus-derived cells. J Clin Invest. 1972; 51(10):2537-43. PMC: 332950. DOI: 10.1172/JCI107069. View

11.

Bentwich Z, Douglas S, Skutelsky E, KUNKEL H . Sheep red cell binding to human lymphocytes treated with neuraminidase; enhancement of T cell binding and identification of a subpopulation of B cells. J Exp Med. 1973; 137(6):1532-7. PMC: 2139353. DOI: 10.1084/jem.137.6.1532. View

12.

Gergely P, Szegedi G, Szabo G, Fedete B, Petranyi G . Letter: Rosette stimulation by plant mitogens. Lancet. 1973; 2(7834):914. DOI: 10.1016/s0140-6736(73)92049-7. View

13.

Klein E, Klein G, NADKARNI J, Nadkarni J, Wigzell H, Clifford P . Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines. Cancer Res. 1968; 28(7):1300-10. View

14.

Baxley G, Bishop G, Cooper A, Wortis H . Rosetting of human red blood cells to thymocytes and thymus-derived cells. Clin Exp Immunol. 1973; 15(3):385-92. PMC: 1553895. View

15.

Hadden J, Hadden E, Haddox M, Goldberg N . Guanosine 3':5'-cyclic monophosphate: a possible intracellular mediator of mitogenic influences in lymphocytes. Proc Natl Acad Sci U S A. 1972; 69(10):3024-7. PMC: 389699. DOI: 10.1073/pnas.69.10.3024. View

16.

Barnett R, Scott R, Furcht L, Kersey J . Evidence that mitogenic lectins induce changes in lymphocyte membrane fluidity. Nature. 1974; 249(456):465-6. DOI: 10.1038/249465a0. View

17.

Chisari F, Edgington T . Human T lymphocyte "E" rosette function. I. A process modulated by intracellular cyclic AMP. J Exp Med. 1974; 140(4):1122-6. PMC: 2139637. DOI: 10.1084/jem.140.4.1122. View

18.

Schumm D, MORRIS H, Webb T . Early biochemical changes in phytohemagglutinin-stimulated peripheral blood lymphocytes from normal and tumor-bearing rats. Eur J Cancer (1965). 1974; 10(2):107-13. DOI: 10.1016/0014-2964(74)90061-9. View

19.

Smith J, Steiner A, NEWBERRY Jr W, Parker C . Cyclic adenosine 3',5'-monophosphate in human lymphocytes. Alterations after phytohemagglutinin stimulation. J Clin Invest. 1971; 50(2):432-41. PMC: 291939. DOI: 10.1172/JCI106510. View

20.

Lozzio C, LOZZIO B . Cytotoxicity of a factor isolated from human spleen. J Natl Cancer Inst. 1973; 50(2):535-8. DOI: 10.1093/jnci/50.2.535. View