Microtubule-associated Protein 2 (MAP2)-immunoreactive Neurons in the Retina of Bufo Marinus: Colocalisation with Tyrosine Hydroxylase and Serotonin in Amacrine Cells

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 1992 Jul 1

PMID 1358449

Citations 7

Authors

R Gabriel

M Wilhelm

C Straznicky

Affiliations

Soon will be listed here.

Abstract

Neuron populations in the retina of the toad, Bufo marinus, were labelled with a monoclonal antibody raised against microtubule-associated protein 2 (MAP2). A subpopulation of cones, probably corresponding to the blue-sensitive small single cones, large diameter amacrine cells in the most proximal row of the inner nuclear layer and some large ganglion cells in the ganglion cell layer were labelled. Double labelling experiments were carried out to establish the colocalisation of MAP2 with known putative transmitter substances of the anuran amacrine cells. MAP2 was colocalised in a subpopulation of serotonin-immunoreactive and in all tyrosine hydroxylase-immunoreactive amacrine cells. The results indicate, that the MAP2 content in the neurons of the anuran retina can be correlated with other well-defined neurochemical and/or physiological properties.

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References

Pasteels B, Parmentier M, Lawson E, Verstappen A, Pochet R . Calcium binding protein immunoreactivity in pigeon retina. Invest Ophthalmol Vis Sci. 1987; 28(4):658-64. View

Szel A, DIAMANTSTEIN T, Rohlich P . Identification of the blue-sensitive cones in the mammalian retina by anti-visual pigment antibody. J Comp Neurol. 1988; 273(4):593-602. DOI: 10.1002/cne.902730413. View

Gabriel R, Straznicky C . GABA-like immunoreactive neurons in the retina of Bufo marinus: evidence for the presence of GABA-containing ganglion cells. Brain Res. 1992; 571(1):175-9. DOI: 10.1016/0006-8993(92)90528-h. View

Zhang Y, Straznicky C . The morphology and distribution of photoreceptors in the retina of Bufo marinus. Anat Embryol (Berl). 1991; 183(1):97-104. DOI: 10.1007/BF00185840. View

Hiscock J, Straznicky C . Neuropeptide Y-like immunoreactive amacrine cells in the retina of Bufo marinus. Brain Res. 1989; 494(1):55-64. DOI: 10.1016/0006-8993(89)90143-1. View

MATUS A . Microtubule-associated proteins: their potential role in determining neuronal morphology. Annu Rev Neurosci. 1988; 11:29-44. DOI: 10.1146/annurev.ne.11.030188.000333. View

Hirokawa N, Hisanaga S, Shiomura Y . MAP2 is a component of crossbridges between microtubules and neurofilaments in the neuronal cytoskeleton: quick-freeze, deep-etch immunoelectron microscopy and reconstitution studies. J Neurosci. 1988; 8(8):2769-79. PMC: 6569399. View

Zhu B, Hiscock J, Straznicky C . The changing distribution of neurons in the inner nuclear layer from metamorphosis to adult: a morphometric analysis of the anuran retina. Anat Embryol (Berl). 1990; 181(6):585-94. DOI: 10.1007/BF00174630. View

Osborne N, Nicholas D, Dockray G, Cuello A . Cholecystokinin and substance P immunoreactivity in retinas of rats, frogs, lizards and chicks. Exp Eye Res. 1982; 34(4):639-49. DOI: 10.1016/0014-4835(82)90038-0. View

10.

Viereck C, Tucker R, Binder L, MATUS A . Phylogenetic conservation of brain microtubule-associated proteins MAP2 and tau. Neuroscience. 1988; 26(3):893-904. DOI: 10.1016/0306-4522(88)90107-8. View

11.

de Monasterio F, McCrane E, Newlander J, Schein S . Density profile of blue-sensitive cones along the horizontal meridian of macaque retina. Invest Ophthalmol Vis Sci. 1985; 26(3):289-302. View

12.

Rohrenbeck J, Wassle H, Boycott B . Horizontal Cells in the Monkey Retina: Immunocytochemical staining with antibodies against calcium binding proteins. Eur J Neurosci. 1989; 1(5):407-420. DOI: 10.1111/j.1460-9568.1989.tb00349.x. View

13.

Rohlich P, Szel A, Papermaster D . Immunocytochemical reactivity of Xenopus laevis retinal rods and cones with several monoclonal antibodies to visual pigments. J Comp Neurol. 1989; 290(1):105-17. DOI: 10.1002/cne.902900107. View

14.

Ahnelt P, Kolb H, Pflug R . Identification of a subtype of cone photoreceptor, likely to be blue sensitive, in the human retina. J Comp Neurol. 1987; 255(1):18-34. DOI: 10.1002/cne.902550103. View

15.

Herzog W, Weber K . Fractionation of brain microtubule-associated proteins. Isolation of two different proteins which stimulate tubulin polymerization in vitro. Eur J Biochem. 1978; 92(1):1-8. DOI: 10.1111/j.1432-1033.1978.tb12716.x. View

16.

Morris J, Gibbins I, Campbell G, Murphy R, Furness J, Costa M . Innervation of the large arteries and heart of the toad (Bufo marinus) by adrenergic and peptide-containing neurons. Cell Tissue Res. 1986; 243(1):171-84. DOI: 10.1007/BF00221866. View

17.

Wassle H, BOYCOTT B . Functional architecture of the mammalian retina. Physiol Rev. 1991; 71(2):447-80. DOI: 10.1152/physrev.1991.71.2.447. View

18.

Kim H, Binder L, Rosenbaum J . The periodic association of MAP2 with brain microtubules in vitro. J Cell Biol. 1979; 80(2):266-76. PMC: 2110341. DOI: 10.1083/jcb.80.2.266. View

19.

DeMonasterio F, Schein S, McCrane E . Staining of blue-sensitive cones of the macaque retina by a fluorescent dye. Science. 1981; 213(4513):1278-81. DOI: 10.1126/science.7268439. View

20.

Witkovsky P, Levine J, Engbretson G, Hassin G, Macnichol Jr E . A microspectrophotometric study of normal and artificial visual pigments in the photoreceptors of Xenopus laevis. Vision Res. 1981; 21(6):867-73. DOI: 10.1016/0042-6989(81)90187-5. View