Regulation of Hypoxanthine Transport in Neurospora Crassa

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1976 Nov 1

PMID 135758

Citations 7

Authors

R L Sabina

J M Magill

C W Magill

Affiliations

Soon will be listed here.

Abstract

Hypoxanthine uptake and hypoxanthine phosphoribosyltransferase activity (EC 2.4.2.8) were determined in germinated conidia from the adenine auxotrophic strains ad-1 and ad-8 and the double mutant strain ad-1 ad-8. The mutant strain ad-1 appears to lack aminoimidazolecarboximide ribonucleotide formyltransferase (EC 2.1.2.3) or inosine 5'monophosphate cyclohydrolase (EC 3.5.1.10) activities, or both, whereas the ad-8 strain lacks adenylosuccinate synthase activity (EC 6.3.4.4). Normal (or wild-type) hypoxanthine transport capacity was found to the ad-1 conidia, whereas the ad-8 strains failed to take up any hypoxanthine. The double mutant strains showed intermediate transport capacities. Similar results were obtained for hypoxanthine phosphoribosyl-transferase activity assayed in germinated conidia. The ad-1 strain showed greatest activity, the ad-8 strain showed the least activity, and the double mutant strain showed intermediate activity levels. Ion-exchange chromatography of the growth media revealed that in the presence of NH+/4, the ad-8 strain excreted hypoxanthine or inosine, the ad-1 strain did not excrete any purines, and the ad-1 ad-8 double mutant strain excreted uric acid. In the absence of NH+/4, none of the strains excreted any detectable purine compounds.

Citing Articles

Guanine ribonucleotide depletion in mammalian cells. A target of purine antimetabolites.

Nguyen B, Cohen M, Sadee W Cancer Chemother Pharmacol. 1983; 11(2):117-9.

PMID: 6578881 DOI: 10.1007/BF00254259.

Chromosomal loci of Neurospora crassa.

Perkins D, Radford A, Newmeyer D, Bjorkman M Microbiol Rev. 1982; 46(4):426-570.

PMID: 6219280 PMC: 281555. DOI: 10.1128/mr.46.4.426-570.1982.

Guanine uptake and metabolism in Neurospora crassa.

Magill C, Sabina R, Garber T, Magill J J Bacteriol. 1982; 149(3):941-7.

PMID: 6174500 PMC: 216481. DOI: 10.1128/jb.149.3.941-947.1982.

Role of purine base excretion in regulation of purine pools.

Sabina R, Hanks A, Magill J, Magill C Mol Gen Genet. 1979; 173(1):31-8.

PMID: 157418 DOI: 10.1007/BF00267688.

Specificity of uracil uptake in Neurospora crassa.

Dalke P, Magill J J Bacteriol. 1979; 139(1):212-9.

PMID: 156718 PMC: 216847. DOI: 10.1128/jb.139.1.212-219.1979.

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