Pertussis Toxin Pretreatment Abolishes the Inhibitory Effect of Riluzole and Carbachol on D-[3H]aspartate Release from Cultured Cerebellar Granule Cells

Overview

Journal Neurosci Lett

Specialty Neurology

Date 1992 Jun 22

PMID 1354343

Citations 14

Authors

A Doble

J P Hubert

J C Blanchard

Affiliations

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Abstract

The release of D-[3H]aspartate from cultured cerebellar granule cells evoked by glutamic acid can be inhibited by riluzole and the muscarinic agonist carbachol. The combined application of maximally efficacious concentrations of riluzole and carbachol produces no greater inhibition than that seen with either agent alone, indicating that a common mechanism is involved. The effects of both agents are abolished when the cells have been pretreated with pertussis toxin, which suggests that this mechanism may involve a GTP-binding protein. The effect of pertussis toxin pretreatment is not mimicked by cholera toxin, nor does pertussis toxin pretreatment interfere with the inhibitory effect of the competitive excitatory amino acid receptor antagonist D-alpha-aminoadipic acid.

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