Calcium-dependent Transmitter Secretion Reconstituted in Xenopus Oocytes: Requirement for Synaptophysin

Overview

Journal Science

Specialty Science

Date 1992 Jul 31

PMID 1353905

Citations 33

Authors

J Alder

B Lu

F Valtorta

P Greengard

M M Poo

Affiliations

Soon will be listed here.

Abstract

Calcium-dependent glutamate secretion was reconstituted in Xenopus oocytes by injecting the oocyte with total rat cerebellar messenger RNA (mRNA). Co-injection of total mRNA with antisense oligonucleotides to synaptophysin message decreased the expression of synaptophysin in the oocyte and reduced the calcium-dependent secretion. A similar effect on secretion was observed for oocytes injected with total mRNA together with an antibody to rat synaptophysin. These results indicate that synaptophysin is necessary for transmitter secretion and that the oocyte expression system may be useful for dissecting the molecular events associated with the secretory process.

Citing Articles

Sprouting sympathetic fibres release CXCL16 and norepinephrine to synergistically mediate sensory neuronal hyperexcitability in a rodent model of neuropathic pain.

Wang C, Di A, Wu Y, Liu M, Wei M, Liang Z Br J Anaesth. 2025; 134(3):804-816.

PMID: 39848871 PMC: 11867076. DOI: 10.1016/j.bja.2024.10.019.

Elevated matrix Metalloproteinase-9 associated with reduced cerebellar perineuronal nets in female mice with toxoplasmosis.

Xiao J, Huang J, Yolken R Brain Behav Immun Health. 2024; 36:100728.

PMID: 38323226 PMC: 10844038. DOI: 10.1016/j.bbih.2024.100728.

Association between genetic polymorphisms of synaptophysin (SYP) gene and attention deficit hyperactivity disorder in Korean subjects.

Kim H, Kim S, Kim G, Jin H Genes Genomics. 2023; 45(9):1097-1105.

PMID: 37133725 DOI: 10.1007/s13258-023-01393-7.

Experience-dependent structural plasticity at pre- and postsynaptic sites of layer 2/3 cells in developing visual cortex.

Sun Y, Espinosa J, Hoseini M, Stryker M Proc Natl Acad Sci U S A. 2019; 116(43):21812-21820.

PMID: 31591211 PMC: 6815154. DOI: 10.1073/pnas.1914661116.

Medial prefrontal cortex exacerbates gastric dysfunction of rats upon restraint water‑immersion stress.

Zhao D, Gong S, Ma Y, Zhu J Mol Med Rep. 2019; 20(3):2303-2315.

PMID: 31322177 PMC: 6691265. DOI: 10.3892/mmr.2019.10462.