Suppression of Efferent Limb of Testicular Autoimmune Response by a Regulatory CD4+ T Cell Line in Mice

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1992 Mar 1

PMID 1347494

Citations 8

Authors

M Itoh

A Mukasa

Y Tokunaga

C Hiramine

K Hojo

Affiliations

Soon will be listed here.

Abstract

A murine T cell line (designated as C.Ts) as a mediator of suppression of experimental autoimmune orchitis (EAO) was established. The method of establishment of C.Ts cell line was preparing spleen cells from C3H/He mice hyperimmunized with testicular germ cells (TC) and the repeated selection of the lymphocytes in vitro by stimulation with mouse testicular antigens (mTA). The C.Ts cells were Thy1.2+, surface immunoglobulin-, CD3+, CD4+ and CD8-. The cells could suppress the induction of EAO when transferred into actively EAO-sensitized mice only at the pre-clinical stage of the disease (efferent limb of the autoimmune response). The transferred C.Ts cells significantly inhibited both cellular and humoral immune responses to TC in the recipients in an antigen-specific manner. The disease suppression by C.Ts cells was found to depend upon their cell number, and their suppressive activity was markedly augmented by in vitro stimulation with mTA.

Citing Articles

GIT1 is an untolerized autoantigen involved in immunologic disturbance of spermatogenesis.

Nagahori K, Kuramasu M, Kawata S, Yakura T, Li Z, Hirai S Histochem Cell Biol. 2022; 157(3):309-319.

PMID: 35066604 DOI: 10.1007/s00418-021-02061-1.

T Lymphocytes and Testicular Immunity: A New Insight into Immune Regulation in Testes.

Gong J, Zeng Q, Yu D, Duan Y Int J Mol Sci. 2020; 22(1).

PMID: 33374605 PMC: 7793097. DOI: 10.3390/ijms22010057.

Immunological microenvironment in the testis.

Qu N, Ogawa Y, Kuramasu M, Nagahori K, Sakabe K, Itoh M Reprod Med Biol. 2020; 19(1):24-31.

PMID: 31956282 PMC: 6955586. DOI: 10.1002/rmb2.12293.

Development of CD4+ T cell lines that suppress an antigen-specific immune response in vivo.

de Moraes L, Sun B, Rizzo L Clin Exp Immunol. 2003; 131(1):17-25.

PMID: 12519381 PMC: 1808607. DOI: 10.1046/j.1365-2249.2003.02018.x.

Essential pathogenic role for endogenous interferon-gamma (IFN-gamma) during disease onset phase of murine experimental autoimmune orchitis. I. In vivo studies.

Itoh M, Yano A, Xie Q, Iwahashi K, Takeuchi Y, Meroni P Clin Exp Immunol. 1998; 111(3):513-20.

PMID: 9528891 PMC: 1904883. DOI: 10.1046/j.1365-2249.1998.00527.x.

References

Itoh M, Hiramine C, Koseto M, Hojo K . Enzyme-linked immunosorbent assay (ELISA) for detecting antisperm antibodies in mice with testicular autoimmunity. Am J Reprod Immunol. 1989; 21(1):9-15. DOI: 10.1111/j.1600-0897.1989.tb00991.x. View

Myers L, Stuart J, Kang A . A CD4 cell is capable of transferring suppression of collagen-induced arthritis. J Immunol. 1989; 143(12):3976-80. View

Hojo K, Hiramine C . In vivo effects of cyclosporin A: abrogation of the induction of experimental allergic orchitis and sparing of the generation of suppressor cells. Int Arch Allergy Appl Immunol. 1985; 78(1):63-70. DOI: 10.1159/000233864. View

Cohen I, Holoshitz J, van Eden W, Frenkel A . T lymphocyte clones illuminate pathogenesis and affect therapy of experimental arthritis. Arthritis Rheum. 1985; 28(8):841-5. DOI: 10.1002/art.1780280802. View

Sakaguchi S, Takahashi T, NISHIZUKA Y . Study on cellular events in post-thymectomy autoimmune oophoritis in mice. II. Requirement of Lyt-1 cells in normal female mice for the prevention of oophoritis. J Exp Med. 1982; 156(6):1577-86. PMC: 2186864. DOI: 10.1084/jem.156.6.1577. View

Hojo K, Hiramine C . Suppression of experimental allergic orchitis and cellular immune response in the guinea pig by pretreatment with testis antigen in incomplete Freund's adjuvant. Int Arch Allergy Appl Immunol. 1982; 69(1):40-9. DOI: 10.1159/000233144. View

Itoh M, Hiramine C, Tokunaga Y, Mukasa A, Hojo K . A new murine model of autoimmune orchitis induced by immunization with viable syngeneic testicular germ cells alone. II. Immunohistochemical findings of fully-developed inflammatory lesion. Autoimmunity. 1991; 10(2):89-97. DOI: 10.3109/08916939109004812. View

Sugihara S, Maruo S, Tsujimura T, TARUTANI O, Kohno Y, Hamaoka T . Autoimmune thyroiditis induced in mice depleted of particular T cell subsets. III. Analysis of regulatory cells suppressing the induction of thyroiditis. Int Immunol. 1990; 2(4):343-51. DOI: 10.1093/intimm/2.4.343. View

Boitard C, Yasunami R, Dardenne M, Bach J . T cell-mediated inhibition of the transfer of autoimmune diabetes in NOD mice. J Exp Med. 1989; 169(5):1669-80. PMC: 2189316. DOI: 10.1084/jem.169.5.1669. View

10.

Karpus W, Swanborg R . CD4+ suppressor cells differentially affect the production of IFN-gamma by effector cells of experimental autoimmune encephalomyelitis. J Immunol. 1989; 143(11):3492-7. View

11.

Perry L, Barzaga M, Ferguson T . Modulation of experimental allergic encephalomyelitis with anti-T suppressor factor antibodies. J Immunol. 1988; 141(9):3016-21. View

12.

Aoki I, Ishii N, Minami M, Nagashima Y, MISUGI K, Okuda K . Induction of suppressor T cells by intravenous administration of monoclonal anti-I-A antibody. Transplantation. 1987; 44(3):421-5. DOI: 10.1097/00007890-198709000-00019. View

13.

Hiramine C, Hojo K . Adoptive transfer of suppression of experimental allergic orchitis with lymphoid cells from antigen-pretreated guinea pigs. Clin Immunol Immunopathol. 1984; 30(2):202-13. DOI: 10.1016/0090-1229(84)90055-2. View

14.

Taguchi O, NISHIZUKA Y . Experimental autoimmune orchitis after neonatal thymectomy in the mouse. Clin Exp Immunol. 1981; 46(2):425-34. PMC: 1536395. View

15.

Teuscher C, Hickey W, Korngold R . Experimental allergic orchitis in mice. V. Resistance to actively induced disease in BALB/cJ substrain mice is mediated by CD4+ T cells. Immunogenetics. 1990; 32(1):34-40. DOI: 10.1007/BF01787326. View

16.

Itoh M, Hiramine C, Hojo K . A new murine model of autoimmune orchitis induced by immunization with viable syngeneic testicular germ cells alone. I. Immunological and histological studies. Clin Exp Immunol. 1991; 83(1):137-42. PMC: 1535462. DOI: 10.1111/j.1365-2249.1991.tb05603.x. View

17.

Ellerman K, Powers J, Brostoff S . A suppressor T-lymphocyte cell line for autoimmune encephalomyelitis. Nature. 1988; 331(6153):265-7. DOI: 10.1038/331265a0. View

18.

Kong Y, Giraldo A, Waldmann H, Cobbold S, Fuller B . Resistance to experimental autoimmune thyroiditis: L3T4+ cells as mediators of both thyroglobulin-activated and TSH-induced suppression. Clin Immunol Immunopathol. 1989; 51(1):38-54. DOI: 10.1016/0090-1229(89)90204-3. View

19.

Sakamoto Y, Nomoto K . Suppression of delayed-type hypersensitivity to syngeneic testicular cells in mice: involvement of suppressor T cells which act at the induction stage. Cell Immunol. 1986; 102(1):168-76. DOI: 10.1016/0008-8749(86)90335-7. View