An Electron Microscope Study of the Morphology and Distribution of the Intracytoplasmic Virus-like Particles of Ehrlich Ascites Tumor Cells

Overview

Journal J Biophys Biochem Cytol

Specialty Cell Biology

Date 1957 Mar 25

PMID 13438899

Citations 19

Authors

W R Adams

A M Prince

Affiliations

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Abstract

Electron microscope studies of eight different sublines of Ehrlich ascites tumor cells which had not, as far as could be determined, come in contact with any known virus, revealed dense particles measuring approximately 55 to 70 mmicro in diameter, both within and attached to the wall of cytoplasmic vesicles identified as the endoplasmic reticulum. All tumor sublines contained significant numbers of particles and revealed no qualitative or quantitative differences in particle morphology or distribution. It is concluded that these structures are a constant morphological component of the Ehrlich ascites tumor and that they probably do not represent contaminating virus. Their morphology and distribution are described, and the possible interpretations of their significance are discussed.

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References

Hamlin A, HARFORD C, Parker E, VAN RAVENSWAAY T . Electron microscopy of HeLa cells infected with adenoviruses. J Exp Med. 1956; 104(3):443-54. PMC: 2136575. DOI: 10.1084/jem.104.3.443. View

Selby C, BIESELE J, GREY C . Electron microscope studies of ascites tumor cells. Ann N Y Acad Sci. 1956; 63(5):748-73. DOI: 10.1111/j.1749-6632.1956.tb50891.x. View

Friedlaender M, Moore D, KOPROWSKI H . Studies with the electron microscope of virus-host relationships in Ehrlich ascites tumor cells. II. The localization and possible development of anopheles A virus within the endoplasmic reticulum of the host cell. J Exp Med. 1955; 102(4):371-8. PMC: 2136518. DOI: 10.1084/jem.102.4.371. View

GAYLORD Jr W, Melnick J . Intracellular forms of pox viruses as shown by the electron microscope (Vaccinia, Ectromelia, Molluscum Contagiosum). J Exp Med. 1953; 98(2):157-72. PMC: 2136285. DOI: 10.1084/jem.98.2.157. View

Bernhard W, Bauer A, Guerin M, Oberling C . [Electron microscopy of virus-like inclusions in mouse mammary epitheliomas]. Bull Assoc Fr Etud Cancer. 1955; 42(2):163-78. View

Morgan C, Rose H, Moore D . Structure and development of viruses observed in the electron microscope. III. Influenza virus. J Exp Med. 1956; 104(2):171-82. PMC: 2136663. DOI: 10.1084/jem.104.2.171. View

Palade G, PORTER K . Studies on the endoplasmic reticulum. I. Its identification in cells in situ. J Exp Med. 1954; 100(6):641-56. PMC: 2136401. DOI: 10.1084/jem.100.6.641. View

Selby C, GREY C, Lichtenberg S, Friend C, Moore A, BIESELE J . Submicroscopic cytoplasmic particles occasionally found in the Ehrlich mouse ascites tumor. Cancer Res. 1954; 14(11):790-4. View

GAYLORD Jr W . Intracellular forms of meningopneumonitis virus. J Exp Med. 1954; 100(6):575-80. PMC: 2136399. DOI: 10.1084/jem.100.6.575. View

10.

Morgan C, ELLISON S, Rose H, Moore D . Structure and development of viruses as observed in the electron microscope. I. Herpes simplex virus. J Exp Med. 1954; 100(2):195-202. PMC: 2136369. DOI: 10.1084/jem.100.2.195. View

11.

Fawcett D, Wilson J . A note on the occurrence of virus-like particles in the spontaneous hepatomas of C3H mice. J Natl Cancer Inst. 1955; 15(5, Suppl.):1505-11. View

12.

Friedlaender M, Moore D, Love R, BROWN R, KOPROWSKI H . Studies with the electron microscope of virus-host relationships in Ehrlich ascites tumor cells. I. The identification and structure of anopheles A virus. J Exp Med. 1955; 102(4):361-70. PMC: 2136519. DOI: 10.1084/jem.102.4.361. View

13.

Bunting H . Close-packed array of virus-like particles within cells of a human skin papilloma. Proc Soc Exp Biol Med. 1953; 84(2):327-32. DOI: 10.3181/00379727-84-20636. View

14.

Kuff E, HOGEBOOM G, Dalton A . Centrifugal, biochemical, and electron microscopic analysis of cytoplasmic particulates in liver homogenates. J Biophys Biochem Cytol. 1956; 2(1):33-54. PMC: 2223954. View

15.

Friedlaender M, Moore D . Occurrence of bodies within endoplasmic reticulum of Ehrlich ascites tumor cells. Proc Soc Exp Biol Med. 1956; 92(4):828-31. DOI: 10.3181/00379727-92-22627. View

16.

GAYLORD Jr W . Virus-like particles associated with the Rous sarcoma as seen in sections of tumor. Cancer Res. 1955; 15(2):80-3. View

17.

Dalton A, FELIX M . Cytologic and cytochemical characteristics of the Golgi substance of epithelial cells of the epididymis in situ, in homogenates and after isolation. Am J Anat. 1954; 94(2):171-207. DOI: 10.1002/aja.1000940202. View

18.

PORTER K . Observations on a submicroscopic basophilic component of cytoplasm. J Exp Med. 1953; 97(5):727-50. PMC: 2136295. DOI: 10.1084/jem.97.5.727. View

19.

Bang F, VELLISTO I, Libert R . Electron microscopic evidence concerning the mammary tumor inciter (virus). I. A study of normal and malignant cells from the mammary gland of mice. Bull Johns Hopkins Hosp. 1956; 98(4):255-85. View

20.

Morgan C, ELLISON S, Rose H, Moore D . Structure and development of viruses observed in the electron microscope. II. Vaccinia and fowl pox viruses. J Exp Med. 1954; 100(3):301-10. PMC: 2136374. DOI: 10.1084/jem.100.3.301. View