Evidence for De Novo Rearrangements of Drosophila Transposable Elements Induced by the Passage to the Cell Culture

Overview

Journal Genetica

Specialties Cell Biology
Genetics

Date 1992 Jan 1

PMID 1337055

Citations 11

Authors

C Di Franco

C Pisano

G ECHALIER

N Junakovic

Affiliations

Soon will be listed here.

Abstract

The genomic distribution and the number of elements of eleven transposon families have been compared by the Southern technique between permanent cultured cells, larval salivary glands and the brains and whole flies of an inbred Drosophila line (inb-c) from which the cells were established. In cultured cells, changes in restriction patterns consistent with various types of rearrangements such as amplification, transposition and excision of the elements of copia, 1731, 412, 297 and mdg-4 transposon families are detected whereas B 104, G and blood elements appear stable. In previous reports these rearrangements were not detected among individuals of the inb-c line or among samples of somatic tissues, or in samples spanning years of maintenance of cultured cells. Hence, we believe that they have been induced de novo during the passage to the cell culture.

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