Herbicide-resistant Indica Rice Plants from IRRI Breeding Line IR72 After PEG-mediated Transformation of Protoplasts

Overview

Journal Plant Mol Biol

Date 1992 Nov 1

PMID 1333295

Citations 19

Authors

S K Datta

K Datta

N Soltanifar

G Donn

I Potrykus

Affiliations

Soon will be listed here.

Abstract

The commercially important Indica rice cultivar Oryza sativa cv. IR72 has been transformed using direct gene transfer to protoplasts. PEG-mediated transformation was done with two plasmid constructs containing either a CaMV 35S promoter/HPH chimaeric gene conferring resistance to hygromycin (Hg) or a CaMV 35S promoter/BAR chimaeric gene conferring resistance to a commercial herbicide (Basta) containing phosphinothricin (PPT). We have obtained so far 92 Hgr and 170 PPTr IR72 plants from protoplasts through selection. 31 Hgr and 70 PPTr plants are being grown in the greenhouse to maturity. Data from Southern analysis and enzyme assays proved that the transgene was stably integrated into the host genome and expressed. Transgenic plants showed complete resistance to high doses of the commercial formulations of PPT.

Citing Articles

The role of nanoparticles in transforming plant genetic engineering: advancements, challenges and future prospects.

Rani N, Kumari K, Hooda V Funct Integr Genomics. 2025; 25(1):23.

PMID: 39841261 DOI: 10.1007/s10142-025-01528-x.

Evaluation of antibacterial properties of nisin peptide expressed in carrots.

Fallah Ziarani M, Tohidfar M, Mirjalili M Sci Rep. 2023; 13(1):22123.

PMID: 38092901 PMC: 10719254. DOI: 10.1038/s41598-023-49466-7.

Selectable Markers to Marker-Free Selection in Rice.

Sharma A, Chouhan A, Bhatt T, Kaur A, Minhas A Mol Biotechnol. 2022; 64(8):841-851.

PMID: 35184267 DOI: 10.1007/s12033-022-00460-w.

Efficient -mediated Transformation of The Elite- Rice Variety Komboka.

Luu V, Stiebner M, Maldonado P, Valdes S, Marin D, Delgado G Bio Protoc. 2021; 10(17):e3739.

PMID: 33659399 PMC: 7842583. DOI: 10.21769/BioProtoc.3739.

Down-Regulation of Gene Through Antisense Approach Reduced the Cyanogenic Glycoside Dhurrin in [ (L.) Moench] to Improve Fodder Quality.

Pandey A, Madhu P, Bhat B Front Nutr. 2019; 6:122.

PMID: 31544105 PMC: 6729101. DOI: 10.3389/fnut.2019.00122.

References

Walters D, Vetsch C, POTTS D, Lundquist R . Transformation and inheritance of a hygromycin phosphotransferase gene in maize plants. Plant Mol Biol. 1992; 18(2):189-200. DOI: 10.1007/BF00034948. View

St Schell J . Transgenic plants as tools to study the molecular organization of plant genes. Science. 1987; 237(4819):1176-83. DOI: 10.1126/science.237.4819.1176. View

Spencer T, Gordon-Kamm W, Daines R, Start W, Lemaux P . Bialaphos selection of stable transformants from maize cell culture. Theor Appl Genet. 2013; 79(5):625-31. DOI: 10.1007/BF00226875. View

Bilang R, Iida S, PETERHANS A, Potrykus I, Paszkowski J . The 3'-terminal region of the hygromycin-B-resistance gene is important for its activity in Escherichia coli and Nicotiana tabacum. Gene. 1991; 100:247-50. DOI: 10.1016/0378-1119(91)90375-l. View

Hayashimoto A, Li Z, Murai N . A polyethylene glycol-mediated protoplast transformation system for production of fertile transgenic rice plants. Plant Physiol. 1990; 93(3):857-63. PMC: 1062601. DOI: 10.1104/pp.93.3.857. View

Davey M, Rech E, Mulligan B . Direct DNA transfer to plant cells. Plant Mol Biol. 1989; 13(3):273-85. DOI: 10.1007/BF00025315. View

Tada Y, Sakamoto M, Matsuoka M, Fujimura T . Expression of a monocot LHCP promoter in transgenic rice. EMBO J. 1991; 10(7):1803-8. PMC: 452854. DOI: 10.1002/j.1460-2075.1991.tb07705.x. View

Hooykaas P . Transformation of plant cells via Agrobacterium. Plant Mol Biol. 1989; 13(3):327-36. DOI: 10.1007/BF00025321. View

Murray M, Thompson W . Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 1980; 8(19):4321-5. PMC: 324241. DOI: 10.1093/nar/8.19.4321. View

10.

Rhodes C, Pierce D, Mettler I, Mascarenhas D, Detmer J . Genetically transformed maize plants from protoplasts. Science. 1988; 240(4849):204-7. DOI: 10.1126/science.2832947. View

11.

Fromm M, Taylor L, Walbot V . Stable transformation of maize after gene transfer by electroporation. Nature. 1986; 319(6056):791-3. DOI: 10.1038/319791a0. View

12.

Gordon-Kamm W, Spencer T, Mangano M, Adams T, Daines R, Start W . Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants. Plant Cell. 1990; 2(7):603-618. PMC: 159915. DOI: 10.1105/tpc.2.7.603. View

13.

Blochlinger K, DIGGELMANN H . Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells. Mol Cell Biol. 1984; 4(12):2929-31. PMC: 369308. DOI: 10.1128/mcb.4.12.2929-2931.1984. View

14.

Negrutiu I, Shillito R, Potrykus I, Biasini G, Sala F . Hybrid genes in the analysis of transformation conditions : I. Setting up a simple method for direct gene transfer in plant protoplasts. Plant Mol Biol. 2013; 8(5):363-73. DOI: 10.1007/BF00015814. View

15.

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

16.

PETERHANS A, Datta S, Datta K, Goodall G, Potrykus I, Paszkowski J . Recognition efficiency of Dicotyledoneae-specific promoter and RNA processing signals in rice. Mol Gen Genet. 1990; 222(2-3):361-8. DOI: 10.1007/BF00633841. View

17.

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

18.

Meijer E, Schilperoort R, Rueb S, Hensgens L . Transgenic rice cell lines and plants: expression of transferred chimeric genes. Plant Mol Biol. 1991; 16(5):807-20. DOI: 10.1007/BF00015073. View

19.

Shillito R, Paszkowski J, Potrykus I . Agarose plating and a bead type culture technique enable and stimulate development of protoplast-derived colonies in a number of plant species. Plant Cell Rep. 2013; 2(5):244-7. DOI: 10.1007/BF00269151. View

20.

Datta K, Potrykus I, Datta S . Efficient fertile plant regeneration from protoplasts of the Indica rice breeding line IR72 (Oryza sativa L.). Plant Cell Rep. 2013; 11(5-6):229-33. DOI: 10.1007/BF00235071. View