Detection of Replicative Form of Hepatitis C Virus RNA in Peripheral Blood Mononuclear Cells

Overview

Journal J Infect Dis

Publisher Oxford University Press

Specialty Infectious Diseases

Date 1992 Nov 1

PMID 1328405

Citations 39

Authors

J T Wang

J C Sheu

J T Lin

T H Wang

D S Chen

Affiliations

Soon will be listed here.

Abstract

Paired samples of plasma and peripheral blood mononuclear cells (PBMC) from 7 patients with posttransfusion hepatitis C were collected and studied for the presence of replicative forms of hepatitis C virus (HCV). RNA was extracted and tested for both positive and negative strands of HCV RNA by polymerase chain reaction. Positive-strand RNA of HCV was found in both plasma and PBMC of all 7 patients. However, negative-strand RNA was found in PBMC of 3 patients while none was found in the plasma. Levels of negative-strand RNA were about 10-100 times less than those of positive-strand RNA by semiquantification with serial dilution of viral cDNA. The results suggest that active infection and replication of HCV in PBMC is present in patients with posttransfusion hepatitis C and implicate the extrahepatic infection of HCV.

Citing Articles

Hepatitis Virus-associated Non-hodgkin Lymphoma: Pathogenesis and Treatment Strategies.

Zhang W, Du F, Wang L, Bai T, Zhou X, Mei H J Clin Transl Hepatol. 2023; 11(5):1256-1266.

PMID: 37577221 PMC: 10412707. DOI: 10.14218/JCTH.2022.00079S.

Next-Generation Sequencing Analysis of CpG Methylation of a Tumor Suppressor Gene SHP-1 Promoter in Stable Cell Lines and HCV-Positive Patients.

Devi P, Engdahl K, Punga T, Bergqvist A Viruses. 2022; 14(11).

PMID: 36366451 PMC: 9695419. DOI: 10.3390/v14112352.

Hepatitis C Virus Core Protein Down-Regulates Expression of Src-Homology 2 Domain Containing Protein Tyrosine Phosphatase by Modulating Promoter DNA Methylation.

Devi P, Ota S, Punga T, Bergqvist A Viruses. 2021; 13(12).

PMID: 34960785 PMC: 8709277. DOI: 10.3390/v13122514.

Extra-hepatic infection of hepatitis C virus in the colon tissue and its relationship with hepatitis C virus pathogenesis.

Hetta H, Mekky M, Khalil N, Mohamed W, El-Feky M, Ahmed S J Med Microbiol. 2016; 65(8):703-712.

PMID: 27166142 PMC: 5756491. DOI: 10.1099/jmm.0.000272.

Analysis of mutations in the core and NS5A genes of hepatitis C virus in non-responder and relapser patients after treatment with Peg-IFN-α and ribavirin.

Kumthip K, Chusri P, Pantip C, Thongsawat S, OBrien A, Maneekarn N Virusdisease. 2016; 27(1):55-62.

PMID: 26925445 PMC: 4758312. DOI: 10.1007/s13337-015-0300-x.