HCV RNA and Antibody to HCV Core Protein in Japanese Patients with Chronic Liver Disease

Overview

Journal Dig Dis Sci

Specialty Gastroenterology

Date 1992 Oct 1

PMID 1327684

Citations 1

Authors

N Yuki

N Hayashi

H Hagiwara

T Takehara

A Kasahara

H Fusamoto

S Manabe

A Takamizawa

T Kamada

H Okayama

Affiliations

Soon will be listed here.

Abstract

We evaluated the prevalence of hepatitis C virus (HCV) RNA and antibody (anti-HCVcore) to the putative HCV core protein in Japanese patients with chronic liver disease. Sera were screened by solid-phase enzyme immunoassay with a recombinant HCV core protein and by the reverse transcription-polymerase chain reaction (RT-PCR) test which directly detects the HCV genome. Anti-HCV core was detected with high titers in 95% (69/73) of chronic non-A, non-B hepatitis, and 94% (65/69) of anti-HCVcore-positive patients had the genome. Anti-HCVcore was also found with lower titers in 24% (10/41) of chronic hepatitis B virus carriers, and three of them had the genome. Only one (3%) of the 35 patients negative for anti-HCVcore tested positive to RT-PCR. These findings indicate the overwhelming prevalence of HCV infection in Japanese patients with chronic non-A, non-B hepatitis and a close relationship between the presence of anti-HCVcore and chronic hepatitis C in this population.

Citing Articles

Hepatitis C: progress and problems.

Cuthbert J Clin Microbiol Rev. 1994; 7(4):505-32.

PMID: 7834603 PMC: 358339. DOI: 10.1128/CMR.7.4.505.

References

Takehara T, Hayashi N, Mita E, Hagiwara H, Ueda K, Katayama K . Detection of the minus strand of hepatitis C virus RNA by reverse transcription and polymerase chain reaction: implications for hepatitis C virus replication in infected tissue. Hepatology. 1992; 15(3):387-90. DOI: 10.1002/hep.1840150305. View

Takamizawa A, Mori C, FUKE I, Manabe S, Murakami S, Fujita J . Structure and organization of the hepatitis C virus genome isolated from human carriers. J Virol. 1991; 65(3):1105-13. PMC: 239876. DOI: 10.1128/JVI.65.3.1105-1113.1991. View

Davis G, BALART L, Schiff E, Lindsay K, Bodenheimer Jr H, Perrillo R . Treatment of chronic hepatitis C with recombinant interferon alfa. A multicenter randomized, controlled trial. N Engl J Med. 1989; 321(22):1501-6. DOI: 10.1056/NEJM198911303212203. View

Di Bisceglie A, MARTIN P, Kassianides C, Lisker-Melman M, Murray L, Waggoner J . Recombinant interferon alfa therapy for chronic hepatitis C. A randomized, double-blind, placebo-controlled trial. N Engl J Med. 1989; 321(22):1506-10. DOI: 10.1056/NEJM198911303212204. View

Hopf U, Moller B, Kuther D, Stemerowicz R, Lobeck H, Walter E . Long-term follow-up of posttransfusion and sporadic chronic hepatitis non-A, non-B and frequency of circulating antibodies to hepatitis C virus (HCV). J Hepatol. 1990; 10(1):69-76. DOI: 10.1016/0168-8278(90)90075-3. View

Yuki N, Hayashi N, Kasahara A, Hagiwara H, Katayama K, Fusamoto H . Hepatitis B virus markers and antibodies to hepatitis C virus in Japanese patients with hepatocellular carcinoma. Dig Dis Sci. 1992; 37(1):65-72. DOI: 10.1007/BF01308344. View

Bruix J, Barrera J, Calvet X, Ercilla G, Costa J, Sanchez-Tapias J . Prevalence of antibodies to hepatitis C virus in Spanish patients with hepatocellular carcinoma and hepatic cirrhosis. Lancet. 1989; 2(8670):1004-6. DOI: 10.1016/s0140-6736(89)91015-5. View

Kiyosawa K, Sodeyama T, Tanaka E, Gibo Y, Yoshizawa K, Nakano Y . Interrelationship of blood transfusion, non-A, non-B hepatitis and hepatocellular carcinoma: analysis by detection of antibody to hepatitis C virus. Hepatology. 1990; 12(4 Pt 1):671-5. DOI: 10.1002/hep.1840120409. View

Okamoto H, Munekata E, Tsuda F, Takahashi K, Yotsumoto S, Tanaka T . Enzyme-linked immunosorbent assay for antibodies against the capsid protein of hepatitis C virus with a synthetic oligopeptide. Jpn J Exp Med. 1990; 60(4):223-33. View

10.

Kuo G, Choo Q, Alter H, Gitnick G, Redeker A, Purcell R . An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis. Science. 1989; 244(4902):362-4. DOI: 10.1126/science.2496467. View

11.

Okamoto H, Okada S, Sugiyama Y, Yotsumoto S, Tanaka T, Yoshizawa H . The 5'-terminal sequence of the hepatitis C virus genome. Jpn J Exp Med. 1990; 60(3):167-77. View

12.

Choo Q, Kuo G, Weiner A, Overby L, Bradley D, Houghton M . Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science. 1989; 244(4902):359-62. DOI: 10.1126/science.2523562. View

13.

Colombo M, Kuo G, Choo Q, Donato M, Del Ninno E, Tommasini M . Prevalence of antibodies to hepatitis C virus in Italian patients with hepatocellular carcinoma. Lancet. 1989; 2(8670):1006-8. DOI: 10.1016/s0140-6736(89)91016-7. View

14.

Saito I, Miyamura T, Ohbayashi A, Harada H, Katayama T, Kikuchi S . Hepatitis C virus infection is associated with the development of hepatocellular carcinoma. Proc Natl Acad Sci U S A. 1990; 87(17):6547-9. PMC: 54573. DOI: 10.1073/pnas.87.17.6547. View

15.

Weiner A, Kuo G, Bradley D, Bonino F, Saracco G, Lee C . Detection of hepatitis C viral sequences in non-A, non-B hepatitis. Lancet. 1990; 335(8680):1-3. DOI: 10.1016/0140-6736(90)90134-q. View

16.

Kwok S, Higuchi R . Avoiding false positives with PCR. Nature. 1989; 339(6221):237-8. DOI: 10.1038/339237a0. View

17.

Hagiwara H, Hayashi N, Mita E, Hiramatsu N, Ueda K, Takehara T . Detection of hepatitis C virus RNA in chronic non-A, non-B liver disease. Gastroenterology. 1992; 102(2):692-4. DOI: 10.1016/0016-5085(92)90122-f. View

18.

Harada S, Watanabe Y, Takeuchi K, Suzuki T, Katayama T, Takebe Y . Expression of processed core protein of hepatitis C virus in mammalian cells. J Virol. 1991; 65(6):3015-21. PMC: 240954. DOI: 10.1128/JVI.65.6.3015-3021.1991. View

19.

Kew M, Houghton M, Choo Q, Kuo G . Hepatitis C virus antibodies in southern African blacks with hepatocellular carcinoma. Lancet. 1990; 335(8694):873-4. DOI: 10.1016/0140-6736(90)90474-j. View

20.

Nasoff M, Zebedee S, Inchauspe G, Prince A . Identification of an immunodominant epitope within the capsid protein of hepatitis C virus. Proc Natl Acad Sci U S A. 1991; 88(12):5462-6. PMC: 51893. DOI: 10.1073/pnas.88.12.5462. View