Expression of Stress-responsive Ubiquitin Genes in Potato Tubers

Overview

Journal Plant Mol Biol

Date 1992 Oct 1

PMID 1327270

Citations 18

Authors

J E Garbarino

D R Rockhold

W R Belknap

Affiliations

Soon will be listed here.

Abstract

The stress-induced expression of four different ubiquitin-encoding cDNAs was characterized in potato tuber tissue. The four clones exhibited differences in both structure and expression. The first cDNA encoded a single ubiquitin unit fused to an 80 amino acid ribosomal extension protein identical to the extension protein from tomato. Accumulation of the fusion transcript was induced by injury or ethylene, but not by heat shock. The three remaining ubiquitin cDNAs encoded polyubiquitins with 6 to 7 ubiquitin repeats. The first polyubiquitin gene was induced by injury, heat, or ethylene treatments. The second was induced also by injury or heat, with limited ethylene-dependent accumulation of transcript. Transcript levels of the third polyubiquitin gene were highest in control tubers and decreased markedly with injury, heat shock, or ethylene treatment. The data demonstrate the independent regulation of the different members of the ubiquitin gene family in response to stress and exogenous ethylene.

Citing Articles

GhVIM28, a negative regulator identified from VIM family genes, positively responds to salt stress in cotton.

Yang Z, Lu X, Wang N, Mei Z, Fan Y, Zhang M BMC Plant Biol. 2024; 24(1):432.

PMID: 38773389 PMC: 11107009. DOI: 10.1186/s12870-024-05156-8.

Current perspectives of ubiquitination and SUMOylation in abiotic stress tolerance in plants.

Singh M, Singh A, Yadav N, Yadav D Front Plant Sci. 2022; 13:993194.

PMID: 36212351 PMC: 9533872. DOI: 10.3389/fpls.2022.993194.

Protective Roles of Cytosolic and Plastidal Proteasomes on Abiotic Stress and Pathogen Invasion.

Sarafat Ali M, Baek K Plants (Basel). 2020; 9(7).

PMID: 32630761 PMC: 7412383. DOI: 10.3390/plants9070832.

MPSR1 is a cytoplasmic PQC E3 ligase for eliminating emergent misfolded proteins in .

Kim J, Cho S, Oh T, Ryu M, Yang S, Kim W Proc Natl Acad Sci U S A. 2017; 114(46):E10009-E10017.

PMID: 29087340 PMC: 5699081. DOI: 10.1073/pnas.1713574114.

Impacts on the metabolome of down-regulating polyphenol oxidase in potato tubers.

Shepherd L, Alexander C, Hackett C, McRae D, Sungurtas J, Verrall S Transgenic Res. 2014; 24(3):447-61.

PMID: 25417184 DOI: 10.1007/s11248-014-9850-8.

References

Ozkaynak E, Finley D, Solomon M, Varshavsky A . The yeast ubiquitin genes: a family of natural gene fusions. EMBO J. 1987; 6(5):1429-39. PMC: 553949. DOI: 10.1002/j.1460-2075.1987.tb02384.x. View

Finley D, Bartel B, Varshavsky A . The tails of ubiquitin precursors are ribosomal proteins whose fusion to ubiquitin facilitates ribosome biogenesis. Nature. 1989; 338(6214):394-401. DOI: 10.1038/338394a0. View

Christensen A, Quail P . Sequence analysis and transcriptional regulation by heat shock of polyubiquitin transcripts from maize. Plant Mol Biol. 2013; 12(6):619-32. DOI: 10.1007/BF00044153. View

Logemann J, Schell J, Willmitzer L . Improved method for the isolation of RNA from plant tissues. Anal Biochem. 1987; 163(1):16-20. DOI: 10.1016/0003-2697(87)90086-8. View

Binet M, Weil J, Tessier L . Structure and expression of sunflower ubiquitin genes. Plant Mol Biol. 1991; 17(3):395-407. DOI: 10.1007/BF00040634. View

Hoffman N, Ko K, Milkowski D, Pichersky E . Isolation and characterization of tomato cDNA and genomic clones encoding the ubiquitin gene ubi3. Plant Mol Biol. 1991; 17(6):1189-201. DOI: 10.1007/BF00028735. View

Crosby J, Vayda M . Stress-Induced Translational Control in Potato Tubers May Be Mediated by Polysome-Associated Proteins. Plant Cell. 1991; 3(9):1013-1023. PMC: 160067. DOI: 10.1105/tpc.3.9.1013. View

Nairn C, Winesett L, Ferl R . Nucleotide sequence of an actin gene from Arabidopsis thaliana. Gene. 1988; 65(2):247-57. DOI: 10.1016/0378-1119(88)90461-1. View

Hershko A . Ubiquitin-mediated protein degradation. J Biol Chem. 1988; 263(30):15237-40. View

10.

Mignery G, Pikaard C, Hannapel D, Park W . Isolation and sequence analysis of cDNAs for the major potato tuber protein, patatin. Nucleic Acids Res. 1984; 12(21):7987-8000. PMC: 320263. DOI: 10.1093/nar/12.21.7987. View

11.

Jackson P, Lark K . Ribosomal RNA synthesis in soybean suspension cultures growing in different media. Plant Physiol. 1982; 69(1):234-9. PMC: 426180. DOI: 10.1104/pp.69.1.234. View

12.

BUTLER W, Cook L, Vayda M . Hypoxic stress inhibits multiple aspects of the potato tuber wound response. Plant Physiol. 1990; 93(1):264-70. PMC: 1062498. DOI: 10.1104/pp.93.1.264. View

13.

Redman K, Rechsteiner M . Identification of the long ubiquitin extension as ribosomal protein S27a. Nature. 1989; 338(6214):438-40. DOI: 10.1038/338438a0. View

14.

Gausing K, Barkardottir R . Structure and expression of ubiquitin genes in higher plants. Eur J Biochem. 1986; 158(1):57-62. DOI: 10.1111/j.1432-1033.1986.tb09720.x. View

15.

BURKE T, Callis J, Vierstra R . Characterization of a polyubiquitin gene from Arabidopsis thaliana. Mol Gen Genet. 1988; 213(2-3):435-43. DOI: 10.1007/BF00339613. View

16.

Levinger L, Varshavsky A . Selective arrangement of ubiquitinated and D1 protein-containing nucleosomes within the Drosophila genome. Cell. 1982; 28(2):375-85. DOI: 10.1016/0092-8674(82)90355-5. View

17.

Rickey T, Belknap W . Comparison of the expression of several stress-responsive genes in potato tubers. Plant Mol Biol. 1991; 16(6):1009-18. DOI: 10.1007/BF00016073. View

18.

Day D, Arron G, Christoffersen R, Laties G . Effect of ethylene and carbon dioxide on potato metabolism: stimulation of tuber and mitochondrial respiration, and inducement of the alternative path. Plant Physiol. 1978; 62(5):820-5. PMC: 1092229. DOI: 10.1104/pp.62.5.820. View

19.

Callis J, Raasch J, Vierstra R . Ubiquitin extension proteins of Arabidopsis thaliana. Structure, localization, and expression of their promoters in transgenic tobacco. J Biol Chem. 1990; 265(21):12486-93. View

20.

Verwoerd T, Dekker B, Hoekema A . A small-scale procedure for the rapid isolation of plant RNAs. Nucleic Acids Res. 1989; 17(6):2362. PMC: 317610. DOI: 10.1093/nar/17.6.2362. View