Protein P4 of the Bacteriophage Phi 6 Procapsid Has a Nucleoside Triphosphate-binding Site with Associated Nucleoside Triphosphate Phosphohydrolase Activity

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1992 Oct 1

PMID 1326667

Citations 17

Authors

P Gottlieb

J Strassman

L Mindich

Affiliations

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Abstract

Bacteriophage phi 6 contains three segments of double-stranded RNA. The procapsid consists of proteins P1, P2, P4, and P7, which are encoded by the viral L segment. cDNA copies of this segment have been cloned into plasmids that direct the production of these proteins, which assemble into polyhedral procapsids. These procapsids are capable of packaging plus-sense phi 6 RNA in the presence of nucleoside triphosphate and synthesizing the complementary minus strand to form double-stranded RNA. In this article, we report the presence of a nucleotide-binding site in protein P4. The viral procapsid and nucleocapsid exhibit a nucleoside triphosphate phosphohydrolase activity that converts nucleoside triphosphates into nucleoside diphosphates.

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