Two Cellular Single-strand-specific DNA-binding Proteins Interact with Two Regions of the Bovine Papillomavirus Type 1 Genome, Including the Origin of DNA Replication

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1992 Oct 1

PMID 1326653

Citations 2

Authors

C Habiger

G Stelzer

U Schwarz

E L Winnacker

Affiliations

Soon will be listed here.

Abstract

We have identified and purified to near homogeneity two specific single-stranded DNA-binding factors (SPSF I and II) with molecular masses of 42 and 39 kDa, respectively, from calf thymus. Gel retention analysis and competition experiments demonstrate that the ubiquitous proteins SPSF I and II specifically interact with single-stranded DNA derived from the minimal in vitro origin of replication of bovine papillomavirus type 1 and a region of the viral genome proposed to be involved in plasmid maintenance. Bovine papillomavirus type 1 proteins do not interfere with DNA binding of SPSF I and II. The exact location of the binding domains of SPSF I and II on the DNA has been determined by methylation interference and T4 DNA polymerase footprinting. A potential cellular binding site for SPSF I and II is the major promoter (P2) of the human c-myc gene.

Citing Articles

Characterization of the single-strand-specific BPV-1 origin binding protein, SPSF I, as the HeLa Pur alpha factor.

Jurk M, Weissinger F, Lottspeich F, Schwarz U, Winnacker E Nucleic Acids Res. 1996; 24(14):2799-806.

PMID: 8759014 PMC: 146002. DOI: 10.1093/nar/24.14.2799.

cis-Acting components of human papillomavirus (HPV) DNA replication: linker substitution analysis of the HPV type 11 origin.

Russell J, Botchan M J Virol. 1995; 69(2):651-60.

PMID: 7815528 PMC: 188625. DOI: 10.1128/JVI.69.2.651-660.1995.

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