Antiparallel Side-by-side Dimeric Motif for Sequence-specific Recognition in the Minor Groove of DNA by the Designed Peptide 1-methylimidazole-2-carboxamide Netropsin

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1992 Aug 15

PMID 1323845

Citations 42

Authors

M Mrksich

W S Wade

T J Dwyer

B H Geierstanger

D E Wemmer

P B Dervan

Affiliations

Soon will be listed here.

Abstract

The designed peptide 1-methylimidazole-2-carboxamide netropsin (2-ImN) binds specifically to the sequence 5'-TGACT-3'. Direct evidence from NMR spectroscopy is presented that this synthetic ligand binds DNA as a 2:1 complex, which reveals that the structure is an antiparallel dimer in the minor groove of DNA. This is in contrast to the 1:1 complexes usually seen with most crescent-shaped minor groove binding molecules targeted toward A+T-rich tracts but reminiscent of a dimeric motif found for distamycin at high concentrations. These results suggest that sequence-dependent groove width may play an important role in allowing an expanded set of DNA binding motifs for synthetic peptides.

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