Elevated Levels of Activator Transposase MRNA Are Associated with High Frequencies of Dissociation Excision in Arabidopsis

Overview

Journal Plant Cell

Publisher Oxford University Press

Specialties Biology
Cell Biology

Date 1992 May 1

PMID 1323366

Citations 54

Authors

J Swinburne

L Balcells

S R Scofield

J D Jones

G Coupland

Affiliations

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Abstract

The Activator (Ac) element of maize is active at a low frequency in Arabidopsis. To determine whether this is due to poor expression of the Ac transposase gene, we obtained and studied 19 Arabidopsis transformants containing fusions of the octopine synthase (ocs), nopaline synthase (nos), cauliflower mosaic virus (CaMV) 35S, or Ac promoters to the transposase open reading frame. These transformants were examined both for their ability to drive excision of a Dissociation (Ds) element from a streptomycin resistance gene and for the abundance of the transposase mRNA. Most transformants containing the CaMV 35S fusion have high levels of transposase transcript and drive high frequencies of somatic and germinal excision. These results demonstrated that Arabidopsis contains all of the host functions required for high frequency excision of Ds. Moreover, transposase mRNA abundance varied about 1000-fold among our transformants; this variation enabled us to demonstrate that for the Ac, ocs, and CaMV 35S fusion, raising the mRNA level is closely correlated with increasing excision frequency. We discuss our data in relation to the behavior of Ac in Arabidopsis, maize, and tobacco.

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References

Haring M, Rommens C, Nijkamp H, Hille J . The use of transgenic plants to understand transposition mechanisms and to develop transposon tagging strategies. Plant Mol Biol. 1991; 16(3):449-61. DOI: 10.1007/BF00023995. View

Dooner H, Belachew A . Transposition Pattern of the Maize Element Ac from the Bz-M2(ac) Allele. Genetics. 1989; 122(2):447-57. PMC: 1203716. DOI: 10.1093/genetics/122.2.447. View

Hehl R, Baker B . Properties of the maize transposable element Activator in transgenic tobacco plants: a versatile inter-species genetic tool. Plant Cell. 1990; 2(8):709-21. PMC: 159924. DOI: 10.1105/tpc.2.8.709. View

Logemann J, Schell J, Willmitzer L . Improved method for the isolation of RNA from plant tissues. Anal Biochem. 1987; 163(1):16-20. DOI: 10.1016/0003-2697(87)90086-8. View

Van Sluys M, Tempe J, Fedoroff N . Studies on the introduction and mobility of the maize Activator element in Arabidopsis thaliana and Daucus carota. EMBO J. 1987; 6(13):3881-9. PMC: 553865. DOI: 10.1002/j.1460-2075.1987.tb02728.x. View

Deblaere R, Bytebier B, De Greve H, Deboeck F, Schell J, Van Montagu M . Efficient octopine Ti plasmid-derived vectors for Agrobacterium-mediated gene transfer to plants. Nucleic Acids Res. 1985; 13(13):4777-88. PMC: 321826. DOI: 10.1093/nar/13.13.4777. View

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

Baker B, Schell J, Lorz H, Fedoroff N . Transposition of the maize controlling element "Activator" in tobacco. Proc Natl Acad Sci U S A. 1986; 83(13):4844-8. PMC: 323839. DOI: 10.1073/pnas.83.13.4844. View

Jones J, Carland F, Maliga P, Dooner H . Visual detection of transposition of the maize element activator (ac) in tobacco seedlings. Science. 1989; 244(4901):204-7. DOI: 10.1126/science.244.4901.204. View

10.

Scofield S, Harrison K, Nurrish S, Jones J . Promoter fusions to the Activator transposase gene cause distinct patterns of Dissociation excision in tobacco cotyledons. Plant Cell. 1992; 4(5):573-82. PMC: 160154. DOI: 10.1105/tpc.4.5.573. View

11.

Laufs J, Wirtz U, Kammann M, Matzeit V, Schaefer S, Schell J . Wheat dwarf virus Ac/Ds vectors: expression and excision of transposable elements introduced into various cereals by a viral replicon. Proc Natl Acad Sci U S A. 1990; 87(19):7752-6. PMC: 54826. DOI: 10.1073/pnas.87.19.7752. View

12.

Coupland G, Plum C, Chatterjee S, Post A, Starlinger P . Sequences near the termini are required for transposition of the maize transposon Ac in transgenic tobacco plants. Proc Natl Acad Sci U S A. 1989; 86(23):9385-8. PMC: 298500. DOI: 10.1073/pnas.86.23.9385. View

13.

Melton D, Krieg P, Rebagliati M, Maniatis T, Zinn K, Green M . Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 1984; 12(18):7035-56. PMC: 320141. DOI: 10.1093/nar/12.18.7035. View

14.

Benfey P, Ren L, Chua N . The CaMV 35S enhancer contains at least two domains which can confer different developmental and tissue-specific expression patterns. EMBO J. 1989; 8(8):2195-202. PMC: 401147. DOI: 10.1002/j.1460-2075.1989.tb08342.x. View

15.

Fusswinkel H, Schein S, Courage U, Starlinger P, Kunze R . Detection and abundance of mRNA and protein encoded by transposable element activator (Ac) in maize. Mol Gen Genet. 1991; 225(2):186-92. DOI: 10.1007/BF00269846. View