In Vivo Function of the Proteasome in the Ubiquitin Pathway

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1992 Aug 1

PMID 1322295

Citations 33

Authors

W Seufert

S Jentsch

Affiliations

Soon will be listed here.

Abstract

A major eukaryotic proteolytic system is known to require the covalent attachment of ubiquitin to substrates prior to their degradation, yet the proteinase involved remains poorly defined. The proteasome, a large conserved multi-subunit protein complex of the cytosol and the nucleus, has been implicated in a variety of cellular functions. It is shown here that a yeast mutant with a defective proteasome fails to degrade proteins which are subject to ubiquitin-dependent proteolysis in wild-type cells. Thus, the proteasome is part of the ubiquitin system and mediates the degradation of ubiquitin-protein conjugates in vivo.

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