A Novel Glycoprotein of Feline Infectious Peritonitis Coronavirus Contains a KDEL-like Endoplasmic Reticulum Retention Signal

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1992 Aug 1

PMID 1321279

Citations 20

Authors

H Vennema

L Heijnen

P J Rottier

M C Horzinek

W J Spaan

Affiliations

Soon will be listed here.

Abstract

A new protein of feline infectious peritonitis coronavirus (FIPV) was discovered in lysates of [35S]cysteine-labeled infected cells. Expression of open reading frame (ORF) 6b of FIPV in recombinant vaccinia virus-infected cells was used to identify it as the 6b protein. Further characterization revealed that it is a novel type of viral glycoprotein whose function is not clear. It is a soluble protein contained in microsomes; its slow export from the cell is caused by the presence of an endoplasmic reticulum (ER) retention signal at the C terminus. This amino acid sequence, KTEL, closely resembles the consensus KDEL signal of soluble resident ER proteins. A mutant 6b protein with the C-terminal sequence KTEV became resistant to digestion by endo-beta-N-acetylglucosaminidase H with a half-time that was reduced threefold. In contrast, a mutant with the sequence KDEL was completely retained in the ER. The FIPV 6b protein is the first example of a viral protein with a functional KDEL-like ER retention signal.

Citing Articles

Molecular Detection of Feline Coronavirus Based on Recombinase Polymerase Amplification Assay.

Kobialka R, Ceruti A, Bergmann M, Hartmann K, Truyen U, Abd El Wahed A Pathogens. 2021; 10(10).

PMID: 34684186 PMC: 8538120. DOI: 10.3390/pathogens10101237.

Immunogenicity and efficacy of mRNA COVID-19 vaccine MRT5500 in preclinical animal models.

Kalnin K, Plitnik T, Kishko M, Zhang J, Zhang D, Beauvais A NPJ Vaccines. 2021; 6(1):61.

PMID: 33875658 PMC: 8055913. DOI: 10.1038/s41541-021-00324-5.

Identification and characterization of a Golgi retention signal in feline coronavirus accessory protein 7b.

Florek D, Ehmann R, Kristen-Burmann C, Lemmermeyer T, Lochnit G, Ziebuhr J J Gen Virol. 2017; 98(8):2017-2029.

PMID: 28758629 PMC: 7212014. DOI: 10.1099/jgv.0.000879.

Characterization of monoclonal antibodies against feline coronavirus accessory protein 7b.

Lemmermeyer T, Lamp B, Schneider R, Ziebuhr J, Tekes G, Thiel H Vet Microbiol. 2016; 184:11-9.

PMID: 26854339 PMC: 7117465. DOI: 10.1016/j.vetmic.2015.12.009.

Mutations in the 3c and 7b genes of feline coronavirus in spontaneously affected FIP cats.

Borschensky C, Reinacher M Res Vet Sci. 2014; 97(2):333-40.

PMID: 25128417 PMC: 7111757. DOI: 10.1016/j.rvsc.2014.07.016.

References

Vennema H, Rijnbrand R, Heijnen L, Horzinek M, Spaan W . Enhancement of the vaccinia virus/phage T7 RNA polymerase expression system using encephalomyocarditis virus 5'-untranslated region sequences. Gene. 1991; 108(2):201-9. PMC: 7131192. DOI: 10.1016/0378-1119(91)90435-e. View

Sturman L, Holmes K, Behnke J . Isolation of coronavirus envelope glycoproteins and interaction with the viral nucleocapsid. J Virol. 1980; 33(1):449-62. PMC: 288560. DOI: 10.1128/JVI.33.1.449-462.1980. View

Cavanagh D, Brian D, Enjuanes L, Holmes K, Lai M, Laude H . Recommendations of the Coronavirus Study Group for the nomenclature of the structural proteins, mRNAs, and genes of coronaviruses. Virology. 1990; 176(1):306-7. PMC: 7130901. DOI: 10.1016/0042-6822(90)90259-t. View

Garwes D, Stewart F, Britton P . The polypeptide of Mr 14,000 of porcine transmissible gastroenteritis virus: gene assignment and intracellular location. J Gen Virol. 1989; 70 ( Pt 9):2495-9. DOI: 10.1099/0022-1317-70-9-2495. View

de Groot R, ter Haar R, Horzinek M, van der Zeijst B . Intracellular RNAs of the feline infectious peritonitis coronavirus strain 79-1146. J Gen Virol. 1987; 68 ( Pt 4):995-1002. DOI: 10.1099/0022-1317-68-4-995. View

Fuerst T, Niles E, Studier F, Moss B . Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc Natl Acad Sci U S A. 1986; 83(21):8122-6. PMC: 386879. DOI: 10.1073/pnas.83.21.8122. View

de Groot R, Andeweg A, Horzinek M, Spaan W . Sequence analysis of the 3'-end of the feline coronavirus FIPV 79-1146 genome: comparison with the genome of porcine coronavirus TGEV reveals large insertions. Virology. 1988; 167(2):370-6. PMC: 7130511. DOI: 10.1016/s0042-6822(88)90097-9. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Mackett M, Smith G, Moss B . General method for production and selection of infectious vaccinia virus recombinants expressing foreign genes. J Virol. 1984; 49(3):857-64. PMC: 255547. DOI: 10.1128/JVI.49.3.857-864.1984. View

10.

Fujiki Y, Hubbard A, Fowler S, Lazarow P . Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum. J Cell Biol. 1982; 93(1):97-102. PMC: 2112113. DOI: 10.1083/jcb.93.1.97. View

11.

Andres D, Rhodes J, Meisel R, Dixon J . Characterization of the carboxyl-terminal sequences responsible for protein retention in the endoplasmic reticulum. J Biol Chem. 1991; 266(22):14277-82. View

12.

Pelham H . The retention signal for soluble proteins of the endoplasmic reticulum. Trends Biochem Sci. 1990; 15(12):483-6. DOI: 10.1016/0968-0004(90)90303-s. View

13.

Vennema H, Heijnen L, Zijderveld A, Horzinek M, Spaan W . Intracellular transport of recombinant coronavirus spike proteins: implications for virus assembly. J Virol. 1990; 64(1):339-46. PMC: 249107. DOI: 10.1128/JVI.64.1.339-346.1990. View

14.

Zagouras P, Rose J . Carboxy-terminal SEKDEL sequences retard but do not retain two secretory proteins in the endoplasmic reticulum. J Cell Biol. 1989; 109(6 Pt 1):2633-40. PMC: 2115906. DOI: 10.1083/jcb.109.6.2633. View

15.

Fuerst T, Earl P, Moss B . Use of a hybrid vaccinia virus-T7 RNA polymerase system for expression of target genes. Mol Cell Biol. 1987; 7(7):2538-44. PMC: 365388. DOI: 10.1128/mcb.7.7.2538-2544.1987. View

16.

Rosenberg A, Lade B, Chui D, Lin S, Dunn J, Studier F . Vectors for selective expression of cloned DNAs by T7 RNA polymerase. Gene. 1987; 56(1):125-35. DOI: 10.1016/0378-1119(87)90165-x. View

17.

Smith A, Boursnell M, Binns M, Brown T, Inglis S . Identification of a new membrane-associated polypeptide specified by the coronavirus infectious bronchitis virus. J Gen Virol. 1990; 71 ( Pt 1):3-11. DOI: 10.1099/0022-1317-71-1-3. View