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Cloning and Sequencing of Escherichia Coli MutR Shows Its Identity to TopB, Encoding Topoisomerase III

Overview
Journal J Bacteriol
Publisher American Society for Microbiology
Specialty Microbiology
Date 1992 Aug 1
PMID 1321123
Citations 22
Authors
M A Schofield
R Agbunag
M L Michaels
J H Miller
Affiliations
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Abstract

We have cloned and sequenced the mutR gene from Escherichia coli, which results in an increased frequency of spontaneous deletions, by using a strain carrying a Tn10 derivative inserted into mutR. The analysis of 1,286 bp of mutR sequence shows that this gene is identical to the topB gene, which encodes topoisomerase III. The increased deletion formation is the first reported phenotype for cells lacking topoisomerase III, and this suggests that topoisomerase III is involved in reactions that normally reduce the levels of spontaneous deletions.

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