Enhanced Membrane Protein Kinase C Activity in Myocardial Ischemia

Overview

Journal Basic Res Cardiol

Specialty Cardiology & Vascular Diseases

Date 1992 Jan 1

PMID 1314557

Citations 9

Authors

M R Prasad

R M Jones

Affiliations

Soon will be listed here.

Abstract

The protein kinase activity in cytosol was similar in control, ischemic, and reperfused hearts; however, a 1.5-fold increase in membrane protein kinase activity was induced by ischemia and reperfusion. The H-7 inhibitable cytosolic protein kinase activity decreased by 40% with 30 min ischemia, while that of membrane fraction increased 1.8-fold. However, the CGS9343B inhibitable protein kinase activity in cytosolic fractions was unaffected by ischemia, while that of membrane increased by about 1.7-fold. These results suggest that myocardial ischemia is associated with enhanced protein kinase C and calmodulin-dependent kinase activities in membrane fraction. Furthermore, the results also suggest a translocation of protein kinase C activity from the cytosol to the membrane. Reperfusion of ischemic myocardium did not result in any further increase of protein kinase C and calmodulin-dependent kinase activities in the membrane. These enhanced protein kinase activities also resulted in an enhanced phosphorylation of endogenous membrane proteins. The creatine kinase released from the heart was increased by both ischemia and reperfusion. Therefore, these results suggest that biochemical cascades of reactions caused by enhanced membrane protein kinase C and calmodulin-dependent kinase activities may contribute to ischemic-reperfusion injury.

Citing Articles

Histamine 3 receptor activation reduces the expression of neuronal angiotensin II type 1 receptors in the heart.

Hashikawa-Hobara N, Chan N, Levi R J Pharmacol Exp Ther. 2011; 340(1):185-91.

PMID: 22011436 PMC: 3251025. DOI: 10.1124/jpet.111.187765.

Ischemic preconditioning and heat shock activate Akt via a focal adhesion kinase-mediated pathway in Langendorff-perfused adult rat hearts.

Wei H, Vander Heide R Am J Physiol Heart Circ Physiol. 2009; 298(1):H152-7.

PMID: 19880666 PMC: 2806132. DOI: 10.1152/ajpheart.00613.2009.

Heat stress activates AKT via focal adhesion kinase-mediated pathway in neonatal rat ventricular myocytes.

Wei H, Vander Heide R Am J Physiol Heart Circ Physiol. 2008; 295(2):H561-8.

PMID: 18539755 PMC: 2519232. DOI: 10.1152/ajpheart.00401.2008.

Interruption of endothelin signaling modifies membrane type 1 matrix metalloproteinase activity during ischemia and reperfusion.

Deschamps A, Zavadzkas J, Murphy R, Koval C, McLean J, Jeffords L Am J Physiol Heart Circ Physiol. 2007; 294(2):H875-83.

PMID: 18065523 PMC: 2663358. DOI: 10.1152/ajpheart.00918.2007.

Dual mechanism of autoregulation of protein kinase C in myocardial ischemia.

Simonis G, Schoen S, Braun M, Lichte S, Marquetant R, Strasser R Mol Cell Biochem. 2006; 295(1-2):121-8.

PMID: 16924416 DOI: 10.1007/s11010-006-9281-9.

References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Kraft A, Anderson W . Phorbol esters increase the amount of Ca2+, phospholipid-dependent protein kinase associated with plasma membrane. Nature. 1983; 301(5901):621-3. DOI: 10.1038/301621a0. View

NISHIZUKA Y . The molecular heterogeneity of protein kinase C and its implications for cellular regulation. Nature. 1988; 334(6184):661-5. DOI: 10.1038/334661a0. View

Hirota K, Hirota T, Aguilera G, Catt K . Hormone-induced redistribution of calcium-activated phospholipid-dependent protein kinase in pituitary gonadotrophs. J Biol Chem. 1985; 260(6):3243-6. View

Prasad M, Engelman R, Otani H, Cordis G, Das D . Enhanced phosphodiesteratic breakdown and turnover of phosphoinositides during reperfusion of ischemic rat heart. Circ Res. 1988; 63(5):930-6. DOI: 10.1161/01.res.63.5.930. View

Tani M, Neely J . Role of intracellular Na+ in Ca2+ overload and depressed recovery of ventricular function of reperfused ischemic rat hearts. Possible involvement of H+-Na+ and Na+-Ca2+ exchange. Circ Res. 1989; 65(4):1045-56. DOI: 10.1161/01.res.65.4.1045. View

Liu J, Casley D, NAYLER W . Ischaemia causes externalization of endothelin-1 binding sites in rat cardiac membranes. Biochem Biophys Res Commun. 1989; 164(3):1220-5. DOI: 10.1016/0006-291x(89)91799-3. View

Dosemeci A, Dhallan R, COHEN N, Lederer W, Rogers T . Phorbol ester increases calcium current and simulates the effects of angiotensin II on cultured neonatal rat heart myocytes. Circ Res. 1988; 62(2):347-57. DOI: 10.1161/01.res.62.2.347. View

Brown J, Buxton I, Brunton L . Alpha 1-adrenergic and muscarinic cholinergic stimulation of phosphoinositide hydrolysis in adult rat cardiomyocytes. Circ Res. 1985; 57(4):532-7. DOI: 10.1161/01.res.57.4.532. View

10.

Hidaka H, Inagaki M, Kawamoto S, Sasaki Y . Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide dependent protein kinase and protein kinase C. Biochemistry. 1984; 23(21):5036-41. DOI: 10.1021/bi00316a032. View

11.

Kim H, Kim D, Ikemoto N, Kranias E . Lack of effects of calcium X calmodulin-dependent phosphorylation on Ca2+ release from cardiac sarcoplasmic reticulum. Biochim Biophys Acta. 1987; 903(2):333-40. DOI: 10.1016/0005-2736(87)90223-9. View

12.

Legssyer A, POGGIOLI J, Renard D, Vassort G . ATP and other adenine compounds increase mechanical activity and inositol trisphosphate production in rat heart. J Physiol. 1988; 401:185-99. PMC: 1191844. DOI: 10.1113/jphysiol.1988.sp017157. View

13.

Corr P, Shayman J, Kramer J, Kipnis R . Increased alpha-adrenergic receptors in ischemic cat myocardium. A potential mediator of electrophysiological derangements. J Clin Invest. 1981; 67(4):1232-6. PMC: 370686. DOI: 10.1172/jci110139. View

14.

Fearon C, TASHJIAN Jr A . Thyrotropin-releasing hormone induces redistribution of protein kinase C in GH4C1 rat pituitary cells. J Biol Chem. 1985; 260(14):8366-71. View

15.

Shen A, Jennings R . Kinetics of calcium accumulation in acute myocardial ischemic injury. Am J Pathol. 1972; 67(3):441-52. PMC: 2032736. View

16.

Caroni P, Carafoli E . Regulation of Ca2+-pumping ATPase of heart sarcolemma by a phosphorylation-dephosphorylation Process. J Biol Chem. 1981; 256(18):9371-3. View

17.

Liu X, Prasad M, Engelman R, Jones R, Das D . Role of iron on membrane phospholipid breakdown in ischemic-reperfused rat heart. Am J Physiol. 1990; 259(4 Pt 2):H1101-7. DOI: 10.1152/ajpheart.1990.259.4.H1101. View

18.

VIGNE P, Breittmayer J, Duval D, Frelin C, Lazdunski M . The Na+/Ca2+ antiporter in aortic smooth muscle cells. Characterization and demonstration of an activation by phorbol esters. J Biol Chem. 1988; 263(17):8078-83. View

19.

NAYLER W, Poole-Wilson P, Williams A . Hypoxia and calcium. J Mol Cell Cardiol. 1979; 11(7):683-706. DOI: 10.1016/0022-2828(79)90381-x. View

20.

Das D, Engelman R, Prasad M, Rousou J, Breyer R, Jones R . Improvement of ischemia-reperfusion-induced myocardial dysfunction by modulating calcium-overload using a novel, specific calmodulin antagonist, CGS 9343B. Biochem Pharmacol. 1989; 38(3):465-71. DOI: 10.1016/0006-2952(89)90386-9. View