Tetrahydrobiopterin-dependent Formation of Endothelium-derived Relaxing Factor (nitric Oxide) in Aortic Endothelial Cells

Overview

Journal Biochem J

Specialty Biochemistry

Date 1992 Jan 15

PMID 1297314

Citations 25

Authors

K Schmidt

E R Werner

B Mayer

H Wachter

W R Kukovetz

Affiliations

Soon will be listed here.

Abstract

Inhibition of tetrahydrobiopterin (H4biopterin) biosynthesis in endothelial cells almost completely abolished the agonist-induced formation of endothelium-derived relaxing factor (EDRF) (NO). This inhibitory effect could be antagonized when H4biopterin biosynthesis was restored by activating a salvage pathway. These data indicate that the formation of EDRF strictly depends on the presence of intracellular H4biopterin, which, in addition to Ca2+, may represent a further physiological and/or pathophysiological regulatory of endothelial NO synthases.

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