Alpha 2.0
Login Register
» Articles » PMID: 12970455

Development and Use of a Vaccinia Virus Neutralization Assay Based on Flow Cytometric Detection of Green Fluorescent Protein

Overview
Journal J Virol
Publisher American Society for Microbiology
Date 2003 Sep 13
PMID 12970455
Citations 59
Authors
Patricia L Earl
Jeffrey L Americo
Bernard Moss
Affiliations
Soon will be listed here.
Abstract

A rapid and sensitive neutralization assay is required to evaluate alternative smallpox vaccines. Here we describe the development and use of a 96-well plate, semi-automated, flow cytometric assay that uses a recombinant vaccinia virus expressing enhanced green fluorescent protein and which would be applicable to other viruses.

Citing Articles

Xmas-NT: A Novel Two-Color Fluorescence Micro Neutralization Test for the Detection of Neutralizing Antibodies Against Monkeypox Virus.

Bodenstein C, Bourquain D, Nitsche A, Grossegesse M J Med Virol. 2025; 97(2):e70246.

PMID: 39977402 PMC: 11841928. DOI: 10.1002/jmv.70246.

Optimizing Microneutralization and IFN-γ ELISPOT Assays to Evaluate Mpox Immunity.

Yu Y, Meza K, Colbert C, Hoft D, Jaunarajs A, Blazevic A Vaccines (Basel). 2025; 13(1).

PMID: 39852806 PMC: 11769350. DOI: 10.3390/vaccines13010027.

Mpox mRNA-1769 vaccine inhibits orthopoxvirus replication at intranasal, intrarectal, and cutaneous sites of inoculation.

Cotter C, Ignacio M, Americo J, Earl P, Mucker E, Frey T NPJ Vaccines. 2024; 9(1):256.

PMID: 39719481 PMC: 11668852. DOI: 10.1038/s41541-024-01052-2.

Neutralization Determinants on Poxviruses.

Riccardo V, Pablo G Viruses. 2023; 15(12).

PMID: 38140637 PMC: 10747254. DOI: 10.3390/v15122396.

Inhibition of vaccinia virus L1 N-myristoylation by the host N-myristoyltransferase inhibitor IMP-1088 generates non-infectious virions defective in cell entry.

Priyamvada L, Kallemeijn W, Faronato M, Wilkins K, Goldsmith C, Cotter C PLoS Pathog. 2022; 18(10):e1010662.

PMID: 36215331 PMC: 9584500. DOI: 10.1371/journal.ppat.1010662.

References
1.
Norbury C, Malide D, Gibbs J, Bennink J, Yewdell J . Visualizing priming of virus-specific CD8+ T cells by infected dendritic cells in vivo. Nat Immunol. 2002; 3(3):265-71. DOI: 10.1038/ni762. View
2.
Whitley R . Smallpox: a potential agent of bioterrorism. Antiviral Res. 2003; 57(1-2):7-12. DOI: 10.1016/s0166-3542(02)00195-x. View
3.
Mackett M, Smith G, Moss B . General method for production and selection of infectious vaccinia virus recombinants expressing foreign genes. J Virol. 1984; 49(3):857-64. PMC: 255547. DOI: 10.1128/JVI.49.3.857-864.1984. View
4.
Anton L, Schubert U, Bacik I, Princiotta M, Wearsch P, Gibbs J . Intracellular localization of proteasomal degradation of a viral antigen. J Cell Biol. 1999; 146(1):113-24. PMC: 2199725. DOI: 10.1083/jcb.146.1.113. View
5.
Carroll M, Moss B . E. coli beta-glucuronidase (GUS) as a marker for recombinant vaccinia viruses. Biotechniques. 1995; 19(3):352-4, 356. View