Comparative Study of Renin Expression in the Coagulating Glands of C57BL/6 and Balb/c Mice

Overview

Journal J Anat

Specialty Anatomy & Histology

Date 1992 Oct 1

PMID 1295871

Authors

Y Kon

D Endoh

A Fukamizu

K Murakami

Y Hashimoto

M Sugimura

Affiliations

Soon will be listed here.

Abstract

The comparative localisation of renin in the genital organs, especially in the coagulating glands of male mice of the strains C57BL/6 and Balb/c, was investigated using immunocytochemical, immunoelectron microscopical and Northern blot techniques. Dot-like reactions for renin, of varying diameters, were detected immunocytochemically in the epithelial cells of coagulating glands of C57BL/6 mice, but not in those of Balb/c mice. In both strains, many electron dense granules differing in content and morphology were observed in the epithelial cells of the coagulating glands. Crystalline materials were sometimes contained in these granules. Colloidal gold particles indicating the presence of renin were detected in the electron dense granules of C57BL/6 mice, in which they showed a heterogeneous distribution and were especially located on the crystalline structure. No positive reaction was detected in these crystalline structures in Balb/c mice. Renin mRNA was detected in the coagulating glands of both C57BL/6 and Balb/c mice by Northern blot analysis. However, the expression in C57BL/6 coagulating glands was stronger than that in Balb/c. These findings suggest that renin is synthesised and released in the coagulating glands.

References

Davis J, Freeman R . Mechanisms regulating renin release. Physiol Rev. 1976; 56(1):1-56. DOI: 10.1152/physrev.1976.56.1.1. View

Eskildsen P . Evidence for local renin formation in the rabbit oviduct. Acta Pathol Microbiol Scand A. 1972; 80(6):743-50. DOI: 10.1111/j.1699-0463.1972.tb00345.x. View

Kon Y, Hashimoto Y, Kitagawa H, Sugimura M, Murakami K . Intracellular production of adrenal renin in the fetal mouse. An immuno-electron microscopical study. J Anat. 1991; 176:23-33. PMC: 1260310. View

Pinet F, Corvol M, Bourguignon J, Corvol P . Isolation and characterization of renin-producing human chorionic cells in culture. J Clin Endocrinol Metab. 1988; 67(6):1211-20. DOI: 10.1210/jcem-67-6-1211. View

Fisher E . Lysosomal nature of juxtaglomerular granules. Science. 1966; 152(3730):1752-3. DOI: 10.1126/science.152.3730.1752. View

Naruse M, Naruse K, Inagaki T, Inagami T . Immunoreactive renin in mouse adrenal gland. Localization in the inner cortical region. Hypertension. 1984; 6(2 Pt 1):275-80. View

Palumbo A, Jones C, Lightman A, Carcangiu M, DeCherney A, Naftolin F . Immunohistochemical localization of renin and angiotensin II in human ovaries. Am J Obstet Gynecol. 1989; 160(1):8-14. DOI: 10.1016/0002-9378(89)90077-x. View

Mizuno K, Hoffman L, McKenzie J, Inagami T . Presence of renin secretory granules in rat adrenal gland and stimulation of renin secretion by angiotensin II but not by adrenocorticotropin. J Clin Invest. 1988; 82(3):1007-16. PMC: 303615. DOI: 10.1172/JCI113657. View

Morris B . Triton WR-1339 injected into rats enters renal renin granules. Am J Physiol. 1988; 255(3 Pt 1):E353-6. DOI: 10.1152/ajpendo.1988.255.3.E353. View

10.

Do Y, Sherrod A, Lobo R, Paulson R, Shinagawa T, Chen S . Human ovarian theca cells are a source of renin. Proc Natl Acad Sci U S A. 1988; 85(6):1957-61. PMC: 279900. DOI: 10.1073/pnas.85.6.1957. View

11.

Kon Y, Hashimoto Y, Kitagawa H, Kudo N, Murakami K . Immunohistochemical demonstration of juxtaglomerular cells in the kidneys of domestic mammals and fowls. Jpn J Vet Res. 1986; 34(2):111-23. View

12.

TAUGNER R, Whalley A, Angermuller S, Buhrle C, HACKENTHAL E . Are the renin-containing granules of juxtaglomerular epithelioid cells modified lysosomes?. Cell Tissue Res. 1985; 239(3):575-87. DOI: 10.1007/BF00219236. View

13.

Nakamaru M, Misono K, Naruse M, Workman R, Inagami T . A role for the adrenal renin-angiotensin system in the regulation of potassium-stimulated aldosterone production. Endocrinology. 1985; 117(5):1772-8. DOI: 10.1210/endo-117-5-1772. View

14.

McKenzie J, Naruse K, Inagami T . The renin-angiotensin system in the rat anterior pituitary: colocalization of renin and angiotensin II in gonadotrophs. Anat Rec. 1985; 212(2):158-66. DOI: 10.1002/ar.1092120209. View

15.

Saruta T, Kaplan N . Adrenocortical steroidogenesis: the effects of prostaglandins. J Clin Invest. 1972; 51(9):2246-51. PMC: 292388. DOI: 10.1172/JCI107033. View

16.

BARAJAS L . The development and ultrastructure of the juxtaglomerular cell granule. J Ultrastruct Res. 1966; 15(3):400-13. DOI: 10.1016/s0022-5320(66)80116-8. View

17.

Gomba S, Soltesz B . Histochemistry of lysosomal enzymes in juxtaglomerular cells. Experientia. 1969; 25(5):513. DOI: 10.1007/BF01900791. View

18.

Shebuski R, Aiken J . Angiotensin II stimulation of renal prostaglandin synthesis elevates circulating prostacyclin in the dog. J Cardiovasc Pharmacol. 1980; 2(5):667-77. DOI: 10.1097/00005344-198009000-00015. View

19.

Parmentier M, Inagami T, Pochet R, Desclin J . Pituitary-dependent renin-like immunoreactivity in the rat testis. Endocrinology. 1983; 112(4):1318-23. DOI: 10.1210/endo-112-4-1318. View

20.

Inagami T, Clemens D, Celio M, Brown A, Sandru L, HERSCHKOWITZ N . Immunohistochemical localization of renin in mouse brain. Neurosci Lett. 1980; 18(1):91-8. DOI: 10.1016/0304-3940(80)90218-9. View