Development of a 5' Nuclease-based Real-time PCR Assay for Quantitative Detection of Cariogenic Dental Pathogens Streptococcus Mutans and Streptococcus Sobrinus

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2003 Sep 6

PMID 12958287

Citations 67

Authors

Akihiro Yoshida

Nao Suzuki

Yoshio Nakano

Miki Kawada

Takahiko Oho

Toshihiko Koga

Affiliations

Soon will be listed here.

Abstract

A 5' nuclease TaqMan PCR assay was developed for the quantitative detection of the major cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. The absolute and relative numbers of bacteria were measured by this method. This assay will be useful for quantifying these organisms in oral specimens and for analyzing biofilm formation.

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