Signature-tagged Mutagenesis of Pasteurella Multocida Identifies Mutants Displaying Differential Virulence Characteristics in Mice and Chickens

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 2003 Aug 23

PMID 12933901

Citations 26

Authors

Marina Harper

John D Boyce

Ian W Wilkie

Ben Adler

Affiliations

Soon will be listed here.

Abstract

Pasteurella multocida is the causative agent of fowl cholera in birds. Signature-tagged mutagenesis (STM) was used to identify potential virulence factors in a mouse septicemia disease model and a chicken fowl cholera model. A library of P. multocida mutants was constructed with a modified Tn916 and screened for attenuation in both animal models. Mutants identified by the STM screening were confirmed as attenuated by competitive growth assays in both chickens and mice. Of the 15 mutants identified in the chicken model, only 5 were also attenuated in mice, showing for the first time the presence of host-specific virulence factors and indicating the importance of screening for attenuation in the natural host.

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