Effect of Gastrin on Differentiation of Rat Intestinal Epithelial Cells in Vitro

Overview

Journal World J Gastroenterol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2003 Aug 15

PMID 12918121

Citations 5

Authors

Zhou Wang

Wei-Wen Chen

Ru-Liu Li

Bin Wen

Jing-Bo Sun

Affiliations

Soon will be listed here.

Abstract

Aim: To investigate the effect of gastrin on differentiation of IEC-6 cell line in vitro.

Methods: IEC-6 cells were incubated with gastrin. On day 7 after treatment, cell morphology was examined by light microscope, and on day 20, the cellular ultrastructures were examined by electron microscope. After exposure to gastrin for 6 hours, villin mRNA was analyzed by reverse transcription-polymerase chain reaction, and on day 7, the expression of villin was examined by immunocytochemical analysis with laser confocal microscope.

Results: After exposure to gastrin, IEC-6 cells showed differentiated phenotypes as villas enterocytes and contained an abundance of plasma, small nuclei with nucleoli, and were arranged regularly. There were numerous microvilli around edge of the cells, and several cells showed columnar structures. Villin mRNA expression in cytoplasm was increased in comparison with control.

Conclusion: Differentiated characteristics of villus enterocytes and phenotypic changes of rat intestinal epithelial cells (IEC-6) are induced by gastrin, and the effects of gastrin are correlated to increased villin expression.

Citing Articles

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Liao Y, Zhang M, Lonnerdal B Genes Nutr. 2012; 8(1):69-78.

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Construction and evaluation of anti-gastrin immunogen based on P64K protein.

Xiong X, Zhao H, Xue C, Zhang W, Yang B, Yao X World J Gastroenterol. 2006; 12(13):2040-6.

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Coexpression of cholecystokinin-B/gastrin receptor and gastrin gene in human gastric tissues and gastric cancer cell line.

Zhou J, Chen M, Zhang Q, Hu J, Wang W World J Gastroenterol. 2004; 10(6):791-4.

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